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CyFlow™ CD9 Biotin

CyFlow™ CD9 Biotin
Antigen: CD9
Alternative Name: MRP-1, P24
Clone: MEM-61
Application: Flow cytometry, Western blot
Format/Fluorochrome: Biotin
Target Species: Human
Field of Interest: Immunophenotyping
Species of Origin: Mouse
Regulatory Status: RUO
Clonality: monoclonal
Isotype: IgG1
Order number: BE166549

For Research Use Only
Not for use in diagnostic or therapeutic procedures.

Concentration 1 mg/ml Quantity 0.1 mg Volume 0.1 ml Immunogen Pre-B cell line... more
CyFlow™ CD9 Biotin
Concentration1 mg/ml
Quantity0.1 mg
Volume0.1 ml
ImmunogenPre-B cell line NALM-6
SpecifityThe mouse monoclonal antibody MEM-61 recognizes an epitope on second extracellular domain (EC2) of CD9 antigen, a 24 kDa transmembrane protein expressed on platelets, monocytes, pre-B lymphocytes, granulocytes and activated T lymphocytes.
Background InformationCD9 belongs to proteins of tetraspanin family that orchestrate cholesterol-associated tetraspanin-enriched signaling microdomains within the plasma membrane, forming complexes with each other as well as with integrins, membrane-anchored growth factors and other proteins. CD9 is involved in cell motility, osteoclastogenesis, neurite outgrowth, myotube formation, and sperm-egg fusion, plays roles in cell attachment and proliferation and is necessary for association of heterologous MHC II molecules on the dendritic cell plasma membrane which is important for effective T cell stimulation. CD9 is also considered as metastasis suppressor in solid tumors.
UsageThe reagent is designed for indirect immunofluorescence analysis by Flow Cytometry and for Western blotting. Suggested working usage is 2.5·µg/ml (for Flow Cytometry). Indicated dilution is recommended starting point for use of this product, but working concentrations should be validated by the investigator.
Storage BufferThe reagent is provided in phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.1% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.

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