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CyFlow™ CD27 Pacific Blue™
Application: | Flow cytometry |
Emission Maximum: | 455 nm |
Alternative Name: | S152, T14, TNFRSF7, TP55 |
Antigen: | CD27 |
Isotype: | IgG2a |
Excitation Maximum: | 400 to 410 nm |
Clonality: | monoclonal |
Laser: | Violet |
Format/Fluorochrome: | Pacific Blue™ |
Field of Interest: | Immunophenotyping |
Source Species: | Mouse |
Regulatory Status: | RUO |
Target Species: | Human |
Clone: | LT27 |
Order number: | CS942171 |
For Research Use Only
Not for use in diagnostic or therapeutic procedures.
HLDA Workshop HLDA V—WS Code T T-CD27.01 Quantity 100 tests Volume 0.4 mL... more
Product information "CyFlow™ CD27 Pacific Blue™"
HLDA Workshop | HLDA V—WS Code T T-CD27.01 |
Quantity | 100 tests |
Volume | 0.4 mL |
Immunogen | Human peripheral blood lymphocytes |
Background Information | CD27 is a transmembrane 55 kDa protein of the nerve growth factor-receptor family, expressed as a disulfide-linked homodimer on mature thymocytes, peripheral blood T cells and a subpopulation of B cells. Activation of T cells via TCR-CD3 complex results in upregulation of CD27 expression on the plasma membrane as well as in the release of its soluble 28-32 kDa form, sCD27, detected in the plasma, urine or spinal fluid. This sCD27 is an important prognostic marker of acute and chronic B cell malignancies. RgpA, a cystein proteinase, although activating T cells through the protease-activated receptors (PARs), degradates CD27 and counteracts T cell activation mediated by CD27 and its ligand CD70. |
Usage | The reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 4·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests. |
Storage Buffer | The reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide and 0.2% (w/v) BSA. |
Storage | Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze. |
Stability | Do not use after expiration date stamped on vial label. |
Safety Data Sheet
Technical Data Sheet
- CyFlow™ CD27 Pacific Blue™ (LT27) AL442895 TDS US,CA EN.pdf
- CyFlow CD27 Pacific Blue (LT27) CS942171 TDS RUO.pdf
- CyFlow CD27 Pacific Blue (LT27) AL442895 TDS ASR EN.pdf
Instruction for Use
- CyFlow CD27 Pacific Blue (LT27) AG181496 IFU CZ CZ.pdf
- CyFlow CD27 Pacific Blue (LT27) CH394723 IFU CZ CZ.pdf
- CyFlow CD27 Pacific Blue (LT27) CH394723 IFU DE DE.pdf
- CyFlow CD27 Pacific Blue (LT27) CH394723 IFU DK DK.pdf
- CyFlow CD27 Pacific Blue (LT27) CH394723 IFU ES ES.pdf
- CyFlow CD27 Pacific Blue (LT27) CH394723 IFU FR FR.pdf
- CyFlow CD27 Pacific Blue (LT27) CH394723 IFU GB EN.pdf
- CyFlow CD27 Pacific Blue (LT27) CH394723 IFU GR EL.pdf
- CyFlow CD27 Pacific Blue (LT27) CH394723 IFU IT IT.pdf
- CyFlow CD27 Pacific Blue (LT27) CH394723 IFU NL NL.pdf
- CyFlow CD27 Pacific Blue (LT27) CH394723 IFU NO NO.pdf
- CyFlow CD27 Pacific Blue (LT27) CH394723 IFU PL PL.pdf
- CyFlow CD27 Pacific Blue (LT27) CH394723 IFU PT PT.pdf
- CyFlow CD27 Pacific Blue (LT27) CH394723 IFU RU RU.pdf
- CyFlow CD27 Pacific Blue (LT27) CH394723 IFU SE SV.pdf
- CyFlow CD27 Pacific Blue (LT27) CH394723 IFU SK SK.pdf
- CyFlow CD27 Pacific Blue (LT27) CH394723 IFU TR TR.pdf
- CyFlow CD27 Pacific Blue (LT27) CH394723 IFU DE DE.pdf
- CyFlow CD27 Pacific Blue (LT27) AG181496 IFU DE DE.pdf
- CyFlow CD27 Pacific Blue (LT27) AG181496 IFU DK DK.pdf
- CyFlow CD27 Pacific Blue (LT27) AG181496 IFU ES ES.pdf
- CyFlow CD27 Pacific Blue (LT27) AG181496 IFU FR FR.pdf
- CyFlow CD27 Pacific Blue (LT27) AG181496 IFU GB EN.pdf
- CyFlow CD27 Pacific Blue (LT27) AG181496 IFU GR EL.pdf
- CyFlow CD27 Pacific Blue (LT27) AG181496 IFU IT IT.pdf
- CyFlow CD27 Pacific Blue (LT27) AG181496 IFU NL NL.pdf
- CyFlow CD27 Pacific Blue (LT27) AG181496 IFU NO NO.pdf
- CyFlow CD27 Pacific Blue (LT27) AG181496 IFU PL PL.pdf
- CyFlow CD27 Pacific Blue (LT27) AG181496 IFU PT PT.pdf
- CyFlow CD27 Pacific Blue (LT27) AG181496 IFU RU RU.pdf
- CyFlow CD27 Pacific Blue (LT27) AG181496 IFU SE SV.pdf
- CyFlow CD27 Pacific Blue (LT27) AG181496 IFU SK SK.pdf
- CyFlow CD27 Pacific Blue (LT27) AG181496 IFU TR TR.pdf
- CyFlow CD27 Pacific Blue (LT27) AG181496 IFU BG BG.pdf
- CyFlow CD27 Pacific Blue (LT27) AG181496 IFU HR HR.pdf
- CyFlow CD27 Pacific Blue (LT27) AG181496 IFU RO RO.pdf
- CyFlow CD27 Pacific Blue (LT27) AG181496 IFU SI SL.pdf
ECDoC
Certificate Of Analysis
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