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CyFlow™ CD28 Pacific Blue™

CyFlow™ CD28 Pacific Blue™
Antigen: CD28
Alternative Name: Tp44
Clone: CD28.2
Application: Flow cytometry
Format/Fluorochrome: Pacific Blue™
Laser: Violet
Target Species: Human, Non-Human Primates
Field of Interest: Immunophenotyping
Species of Origin: Mouse
Regulatory Status: RUO
Clonality: monoclonal
Emission Maximum: 455 nm
Excitation Maximum: 400 to 410 nm
Isotype: IgG1
Order number: BS327469

For Research Use Only
Not for use in diagnostic or therapeutic procedures.

Description TDS & SDS
Description
Quantity 100 tests Volume 0.4 ml Immunogen DC28.1.3.3 murine T cell hybridoma... more
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CyFlow™ CD28 Pacific Blue™
Quantity100 tests
Volume0.4 ml
ImmunogenDC28.1.3.3 murine T cell hybridoma transfected with human CD28 cDNA
SpecifityThe mouse monoclonal antibody CD28.2 recognizes CD28 antigen, a disulfide-linked homodimeric type I glycoprotein (monomer of Mw 44 kDa) which is a critical costimulatory receptor of T cells.
Background InformationCD28 is the critical T cell costimulatory receptor which provides to the cell the important second activation signal by binding CD80 and CD86 that are expressed by antigen presenting cells. Besides its costimulation role CD28 functions in preventing T cells from anergic hyporesponsive state or from undergoing premature apoptotic cell death. CD28 is also expressed on human fetal NK cells and some NK cell lines, whereas on murine NK cells the CD28 expression is much broader.
UsageThe reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 4·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Storage BufferThe reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.1% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.

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