CyFlow™ CD29 PE

Antigen:	CD29
Alternative Name:	Integrin β1
Clone:	MEM-101A
Application:	Flow cytometry
Format/Fluorochrome:	PE
Laser:	Blue, Green, Yellow
Target Species:	Dog, Human, Pig
Field of Interest:	Immunophenotyping
Species of Origin:	Mouse
Regulatory Status:	RUO
Clonality:	monoclonal
Emission Maximum:	576 nm
Excitation Maximum:	496 nm, 565 nm
Isotype:	IgG1
Order number:	CC990207

For Research Use Only
Not for use in diagnostic or therapeutic procedures.

HLDA Workshop HLDA VI—WS Code AS A048 Quantity 100 tests Volume 2 Immunogen... more

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CyFlow™ CD29 PE

HLDA Workshop	HLDA VI—WS Code AS A048
Quantity	100 tests
Volume	2
Immunogen	Raji cells: human Burkitt's lymphoma cell line
Background Information	CD29 (β1 integrin subunit, GPIIa) forms non-covalently linked heterodimers with at least 6 different α chains (α1-α6, CD49a-f) determining the binding properties of β1 (VLA) integrins. These integrins mediate cell adhesion to collagen, fibronectin, laminin and other extracellular matrix (ECM) components. This interaction hinders cell death, whereas disruption of anchorage to ECM leads to apoptosis. Decreased expression of most β1 integrins correlates with acquiring multidrug resistance of tumor cells during selection in presence of antitumor drug. In platelets, translocation of intracellular pool of β1 integrins to the plasma membrane following thrombin stimulation. These integrins are also up-regulated in leukocytes during emigration and extravascular migration and appear to be critically involved in regulating the immune cell trafficking from blood to tissue, as well as in regulating tissue damage and disease symptoms related to inflammatory bowel disease. Through a β1 integrin-dependent mechanism, fibronectin and type I collagen enhance cytokine secretion of human airway smooth muscle in response to IL-1β.
Usage	The reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 20·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.
Storage Buffer	The reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide.
Storage	Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
Stability	Do not use after expiration date stamped on vial label.

Specific References

| Wencel-Drake JD, Dieter MG, Lam SC: Immunolocalization of beta 1 integrins in platelets and platelet‑derived microvesicles. Blood. 1993·Aug·15; 82(4):1197‑203. <·PMID:·7688992·> | Kishimoto T, Goyert S, Kikutani H, Mason D, Miyasaka M, Moretta L, Ohno T, Okumura K, Shaw S, Springer TA, Sugamura K, Sugawara H, von dem Borne AEGK, Zola H (Eds): Leucocyte Typing VI. Garland·Publishing·Inc,·New·York. 1997; 1‑1342. <·NLM·ID:·9712219·> | Werr J, Johansson J, Eriksson EE, Hedqvist P, Ruoslahti E, Lindbom L: Integrin alpha(2)beta(1 (VLA‑2 is a principal receptor used by neutrophils for locomotion in extravascular tissue. Blood. 2000·Mar·1; 95(5):1804‑9. <·PMID:·10688841·> | Peng Q, Lai D, Nguyen TT, Chan V, Matsuda T, Hirst SJ: Multiple beta 1 integrins mediate enhancement of human airway smooth muscle cytokine secretion by fibronectin and type I collagen. J·Immunol. 2005·Feb·15; 174(4):2258‑64. <·PMID:·15699160·> | Lundberg S, Lindholm J, Lindbom L, Hellström PM, Werr J: Integrin alpha2beta1 regulates neutrophil recruitment and inflammatory activity in experimental colitis in mice. Inflamm·Bowel·Dis. 2006·Mar; 12(3):172‑7. <·PMID:·16534417·> | Morozevich GE, Kozlova NI, Preobrazhenskaya ME, Ushakova NA, Eltsov IA, Shtil AA, Berman AE: The role of beta1 integrin subfamily in anchorage‑dependent apoptosis of breast carcinoma cells differing in multidrug resistance. Biochemistry·(Mosc). 2006·May; 71(5):489‑95. <·PMID:·16732726·> | Simova S, Klima M, Cermak L, Sourkova V, Andera L: Arf and Rho GAP adapter protein ARAP1 participates in the mobilization of TRAIL‑R1/DR4 to the plasma membrane. Apoptosis. 2008·Mar; 13(3):423‑36. <·PMID:·18165900·> | Plánka L, Necas A, Srnec R, Rauser P, Starý D, Jancár J, Amler E, Filová E, Hlucilová J, Kren L, Gál P: Use of allogenic stem cells for the prevention of bone bridge formation in miniature pigs. Physiol·Res. 2009; 58(6):885‑93. <·PMID:·19093735·>

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