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CyFlow™ CD30 PE

CyFlow™ CD30 PE
Application: Flow cytometry
Excitation Maximum: 496 nm, 565 nm
Alternative Name: D1S166E, Ki-1, TNFRSF8
Antigen: CD30
Isotype: IgG1
Emission Maximum: 576 nm
Laser: Blue, Green, Yellow
Clonality: monoclonal
Format/Fluorochrome: PE
Field of Interest: Immunophenotyping
Source Species: Mouse
Regulatory Status: RUO
Target Species: Human
Clone: Ber-H8
Order number: BT330515
HLDA Workshop HLDA III—WS Code A171 HLDA IV—WS Code A105 HLDA V—WS Code A042 Quantity 100... more
Product information "CyFlow™ CD30 PE"
HLDA WorkshopHLDA III—WS Code A171 HLDA IV—WS Code A105 HLDA V—WS Code A042
Quantity100 tests
Volume1.0 mL
ImmunogenNot available
Background InformationCD30 is a type I transmembrane glycoprotein of the TNF receptor superfamily. CD30 was originally identified as a cell surface antigen of Hodgkins and Reed-Sternberg cells using monoclonal antibody Ki-1. The ligand for CD30 is CD30L (CD153). The binding of CD30 to CD30L mediates pleiotropic effects including cell proliferation, activation, differentiation, and apoptotic cell death. CD30 has a critical role in the pathophysiology of Hodgkin's disease and other CD30+ lymphomas. CD30 acts as a costimulatory molecule in thymic negative selection. In addition to its expression on Hodgkin's and Reed-Sternberg cells, CD30 is also found in some non-Hodgkin's lymphomas (including Burkitt's lymphomas), virus-infected T and B cells, and on normal T and B cells after activation. In T cells, CD30 expression is present on a subset of T cells that produce Th2-type cytokines and on CD4+/CD8+ thymocytes that co-express CD45RO and the IL4 receptor. Soluble form of CD30 (sCD30) serves as a marker reflecting Th2 immune response.
UsageThe reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 10·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
Storage BufferThe reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide and 0.2% (w/v) BSA.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.
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