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CyFlow™ CD34 Alexa Fluor™ 488

CyFlow™ CD34 Alexa Fluor™ 488
Application: Flow cytometry
Clonality: monoclonal
Target Species: Human
Regulatory Status: RUO
Laser: Blue
Source Species: Mouse
Field of Interest: Immunophenotyping
Isotype: IgG1
Format/Fluorochrome: Alexa Fluor™ 488
Excitation Maximum: 495 nm
Emission Maximum: 519 nm
Antigen: CD34
Clone: 4H11[APG]
Order number: CX896258
HLDA Workshop HLDA VI—WS Code M MA58 Quantity 100 tests Volume 0.4 mL Immunogen... more
Product information "CyFlow™ CD34 Alexa Fluor™ 488"
HLDA WorkshopHLDA VI—WS Code M MA58
Quantity100 tests
Volume0.4 mL
ImmunogenPermanent human cell line derived from peripheral leucocytes of a patient suffering from chronic myeloid leukaemia
Background InformationCD34 (Mucosialin) is a highly glycosylated monomeric 111-115 kDa surface protein, which is present on many stem cell populations. It is a well established stem cell marker, though its expression on human hematopoietic stem cells is reversible. CD34 probably serves as a surface receptor that undergoes receptor-mediated endocytosis and regulates adhesion, differentiation and proliferation of hematopoietic stem cells and other progenitors. CD34 expression is likely to represent a specific state of hematopoietic development that may have altered adhering properties with expanding and differentiating capabilities in both in vitro and in vivo conditions.
UsageThe reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 4·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Storage BufferThe reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide and 0.2% (w/v) BSA.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.
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