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CyFlow™ CD43 APC-Cy7

CyFlow™ CD43 APC-Cy7
Application: Flow cytometry
Clonality: monoclonal
Target Species: Human
Regulatory Status: RUO
Source Species: Mouse
Field of Interest: Immunophenotyping
Laser: Red
Isotype: IgG1
Format/Fluorochrome: APC-Cy7
Excitation Maximum: 650 nm, 750 nm
Emission Maximum: 780 nm
Antigen: CD43
Alternative Name: Leukosialin, sialophorin
Clone: MEM-59
Order number: CQ526140
HLDA Workshop HLDA IV—WS Code N 604 HLDA V—WS Code AS S290 Quantity 100 tests Volume... more
Product information "CyFlow™ CD43 APC-Cy7"
HLDA WorkshopHLDA IV—WS Code N 604 HLDA V—WS Code AS S290
Quantity100 tests
Volume0.4 mL
ImmunogenHuman T lymphocytes
Background InformationCD43 (leukosialin, sialophorin) is a transmembrane mucin-like protein with high negative charge, expressed on the surface of most hematopoietic cells. CD43 contributes to a repulsive barrier that interferes with cellular adhesion, however, in certain cases also promotes leukocyte aggregation. By interaction with actin-binding proteins ezrin and moesin, CD43 plays a regulatory role in remodeling T-cell morphology and regulates cell-cell interactions during lymphocyte traffic. CD43 signaling both enhances LFA-1 adhesiveness and counteracts LFA-1 induction via other receptors. Expression of CD43 causes induction of functionally active tumor suppressor p53 protein, but in case of p53 and ARF defficiency CD43 promotes tumour proliferation and viability. It appears to be an important modulator of leukocyte functions.
UsageThe reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 4·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Storage BufferThe reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide and 0.2% (w/v) BSA.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.

Safety Data Sheet

Technical Data Sheet

Certificate Of Analysis

ECDoC

Instruction for Use

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