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CyFlow™ CD45 PE

CyFlow™ CD45 PE
Application: Flow cytometry
Excitation Maximum: 496 nm, 565 nm
Clone: MEM-28
Alternative Name: B220, LCA, T200
Antigen: CD45
Isotype: IgG1
Emission Maximum: 576 nm
Laser: Blue, Green, Yellow
Clonality: monoclonal
Format/Fluorochrome: PE
Field of Interest: Immunophenotyping, Phosphorylation
Source Species: Mouse
Regulatory Status: RUO
Target Species: Human
Order number: BE215145

For Research Use Only
Not for use in diagnostic or therapeutic procedures.

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Description TDS & SDS
Description
Quantity 100 tests Volume 2.0 mL Immunogen Human thymocytes and T lymphocytes... more
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Product information "CyFlow™ CD45 PE"
Quantity100 tests
Volume2.0 mL
ImmunogenHuman thymocytes and T lymphocytes
Background InformationCD45 (LCA; leukocyte common antigen) is a receptor-type protein tyrosine phosphatase ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms as a result of alternative splicing; these isoforms differ in their extracellular domains, whereas they share identical transmembrane and cytoplasmic domains. These isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. Besides the role in immunoreceptor signaling, CD45 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis.
UsageThe reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 20·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.
Storage BufferThe reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide and 0.2% (w/v) BSA.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.
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ECDoC

Certificate Of Analysis

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