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CyFlow™ CD45RA Pacific Orange™

CyFlow™ CD45RA Pacific Orange™
Antigen: CD45RA
Alternative Name: Leu18
Clone: MEM-56
Application: Flow cytometry
Format/Fluorochrome: Pacific Orange™
Laser: Violet
Target Species: Human
Field of Interest: Immunophenotyping, Phosphorylation
Species of Origin: Mouse
Regulatory Status: RUO
Clonality: monoclonal
Emission Maximum: 551 nm
Excitation Maximum: 400 nm
Isotype: IgG2b
Order number: CF345450

For Research Use Only
Not for use in diagnostic or therapeutic procedures.

Quantity 100 tests Volume 0.4 ml Immunogen Human thymocytes and T lymphocytes... more
CyFlow™ CD45RA Pacific Orange™
Quantity100 tests
Volume0.4 ml
ImmunogenHuman thymocytes and T lymphocytes
SpecifityThe mouse monoclonal antibody MEM-56 recognizes CD45RA antigen, a 205-220 kDa single chain type I glycoprotein, isoform of the leukocyte common antigen (LCA). CD45RA is expressed on most of B lymphocytes, resting and native T lymphocytes, medullar thymocytes and monocytes.
Background InformationCD45RA is a high molecular weight isoform of a receptor-type protein tyrosine phosphatase, CD45 glycoprotein. CD45 is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases, promotes cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. CD45 isoforms differ in their extracellular domains, whereas they share identical transmembrane and cytoplasmic domains. These isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. CD45RA is expressed e.g. on naive T cells and normal plasma cells.
UsageThe reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 4·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Storage BufferThe reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.1% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.

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