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CyFlow™ CD45RA Pacific Orange™

CyFlow™ CD45RA Pacific Orange™
Antigen: CD45RA
Alternative Name: Leu18
Clone: MEM-56
Application: Flow cytometry
Format/Fluorochrome: Pacific Orange™
Laser: Violet
Target Species: Human
Field of Interest: Immunophenotyping, Phosphorylation
Species of Origin: Mouse
Regulatory Status: RUO
Clonality: monoclonal
Emission Maximum: 551 nm
Excitation Maximum: 400 nm
Isotype: IgG2b
Order number: CF345450

For Research Use Only
Not for use in diagnostic or therapeutic procedures.

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EUROBIOTECH2017

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*1 Offer valid until 23/09/2017
HLDA Workshop HLDA IV—WS Code NL 907 Quantity 100 tests Volume 0.4 Immunogen... more
CyFlow™ CD45RA Pacific Orange™
HLDA WorkshopHLDA IV—WS Code NL 907
Quantity100 tests
Volume0.4
ImmunogenHuman thymocytes and T lymphocytes
Background InformationCD45RA (Leu18) is a high molecular weight isoform of a receptor-type protein tyrosine phosphatase, CD45 glycoprotein. CD45 is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases, promotes cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. CD45 isoforms differ in their extracellular domains, whereas they share identical transmembrane and cytoplasmic domains. These isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. CD45RA is expressed e.g. on naive T cells and normal plasma cells.
UsageThe reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 4·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Storage BufferThe reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.

Specific References

| Bazil V, Horejsi V, Baudys M, Kristofova H, Strominger JL, Kostka W, Hilgert I: Biochemical characterization of a soluble form of the 53‑kDa monocyte surface antigen. Eur·J·Immunol. 1986·Dec; 16(12):1583‑9. <·PMID:·3493149·> | Knapp W, Dorken B, Gilks W, Rieber EP, Schmidt RE, Stein H, von dem Borne AEGK (Eds): Leucocyte Typing IV. Oxford·University·Press,·Oxford. 1989; 1‑1820. <·NLM·ID:·8914679·> | Bhunia AK, Arai T, Bulkley G, Chatterjee S: Lactosylceramide mediates tumor necrosis factor‑alpha‑induced intercellular adhesion molecule‑1 (ICAM‑1 expression and the adhesion of neutrophil in human umbilical vein endothelial cells. J·Biol·Chem. 1998·Dec·18; 273(51):34349‑57. <·PMID:·9852101·> | Dawes R, Petrova S, Liu Z, Wraith D, Beverley PC, Tchilian EZ: Combinations of CD45 isoforms are crucial for immune function and disease. J·Immunol. 2006·Mar·15; 176(6):3417‑25. <·PMID:·16517710·> | Cortez-Gonzalez X, Sidney J, Adotevi O, Sette A, Millard F, Lemonnier F, Langlade-Demoyen P, Zanetti M: Immunogenic HLA‑B7‑restricted peptides of hTRT. Int·Immunol. 2006·Dec; 18(12):1707‑18. <·PMID:·17077179·> | Li C, Wong P, Pan T, Xiao F, Yin S, Chang B, Kang SC, Ironside J, Sy MS: Normal cellular prion protein is a ligand of selectins: binding requires Le(X) but is inhibited by sLe(X). Biochem·J. 2007·Sep·1; 406(2):333‑41. <·PMID:·17497959·> | Desharnais P, Dupéré-Minier G, Hamelin C, Devine P, Bernier J: Involvement of CD45 in DNA fragmentation in apoptosis induced by mitochondrial perturbing agents. Apoptosis. 2008·Feb; 13(2):197‑212. <·PMID:·18157742·>

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