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CyFlow™ CD45RA APC

CyFlow™ CD45RA APC
Application: Flow cytometry
Clonality: monoclonal
Target Species: Human
Regulatory Status: RUO
Isotype: IgG2b
Source Species: Mouse
Field of Interest: Immunophenotyping, Phosphorylation
Format/Fluorochrome: APC
Laser: Red
Excitation Maximum: 650 nm
Emission Maximum: 660 nm
Antigen: CD45RA
Alternative Name: Leu18
Clone: MEM-56
Order number: AH648116
HLDA Workshop HLDA IV—WS Code NL 907 Quantity 100 tests Volume 1.0 mL Immunogen... more
Product information "CyFlow™ CD45RA APC"
HLDA WorkshopHLDA IV—WS Code NL 907
Quantity100 tests
Volume1.0 mL
ImmunogenHuman thymocytes and T lymphocytes
Background InformationCD45RA (Leu18) is a high molecular weight isoform of a receptor-type protein tyrosine phosphatase, CD45 glycoprotein. CD45 is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases, promotes cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. CD45 isoforms differ in their extracellular domains, whereas they share identical transmembrane and cytoplasmic domains. These isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. CD45RA is expressed e.g. on naive T cells and normal plasma cells.
UsageThe reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 10·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
Storage BufferThe reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide and 0.2% (w/v) BSA.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.
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