Welcome to
Sysmex Flow Cytometry Other European Country!

For ordering please make sure you are in your local shop.
If you are from Other European Country then please continue.
Otherwise please go to your country.

Go to my country Continue

CyFlow™ CD45RB Purified

CyFlow™ CD45RB Purified
Antigen: CD45RB
Clone: MEM-143
Application: Flow cytometry, Immunohistochmistry (paraffin-embedded sections)
Format/Fluorochrome: Purified
Target Species: Human
Field of Interest: Immunophenotyping, Phosphorylation
Species of Origin: Mouse
Regulatory Status: RUO
Clonality: monoclonal
Isotype: IgG1
Order number: CS740679

For Research Use Only
Not for use in diagnostic or therapeutic procedures.

Concentration 1 mg/ml Quantity 0.1 mg Volume 0.1 ml Immunogen Human peripheral... more
CyFlow™ CD45RB Purified
Concentration1 mg/ml
Quantity0.1 mg
Volume0.1 ml
ImmunogenHuman peripheral blood lymphocytes
SpecifityThe mouse monoclonal antibody MEM-143 recognizes a protein determinant of CD45RB, a 180-240 kDa single chain type I membrane glycoprotein, variant of CD45 (CD45RB isoform). CD45RB is expressed on a subset of T lymphocytes, B lymphocytes, monocytes, macrophages, granulocytes and dendritic cells. The MEM-143 is therefore not neuraminidase sensitive like most other anti-CD45RB antibodies (including standard antibody MEM-55). The reactivity of the antibody MEM-143 can be blocked by a peptide including amino acids 79-88.
Background InformationCD45RB is a receptor-type protein tyrosine phosphatase, CD45 glycoprotein. CD45 is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases, promotes cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. CD45 isoforms differ in their extracellular domains, whereas they share identical transmembrane and cytoplasmic domains. These isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. CD45RB is expressed e.g. in microglia and inflammatory cells.
Storage BufferThe reagent is provided in phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.1% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.

Article successfully added.