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CyFlow™ CD54 FITC
| Application: | Flow cytometry |
| Clonality: | monoclonal |
| Format/Fluorochrome: | FITC |
| Target Species: | Human |
| Regulatory Status: | RUO |
| Isotype: | IgG2b |
| Laser: | Blue |
| Excitation Maximum: | 490 to 495 nm |
| Emission Maximum: | 518 nm |
| Source Species: | Mouse |
| Field of Interest: | Immunophenotyping |
| Antigen: | CD54 |
| Alternative Name: | ICAM-1 |
| Clone: | 1H4 |
| Order number: | BQ603568 |
For Research Use Only
Not for use in diagnostic or therapeutic procedures.
Quantity 100 tests Volume 2.0 mL Immunogen Raji cells and spleen cells fused with NS1... more
Product information "CyFlow™ CD54 FITC"
| Quantity | 100 tests |
| Volume | 2.0 mL |
| Immunogen | Raji cells and spleen cells fused with NS1 cells |
| Background Information | CD54 (ICAM-1) is a 90 kD member of the C2 subset of immunoglobulin superfamily. It is a transmembrane molecule with 7 potential N-glycosylated sites, expressed on resting monocytes and endothelial cells and can be upregulated on many other cells, e.g. with lymphokines, on B- and T-lymphocytes, thymocytes, dendritic cells and also on keratinocytes, chondrocytes, as well as epithelial cells. CD54 mediates cell adhesion by binding to integrins CD11a/CD18 (LFA-1) and to CD11b/CD18 (Mac-1). The interaction of CD54 with LFA-1 enhances antigen-specific T-cell activation. |
| Usage | The reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 20·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests. |
| Storage Buffer | The reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide and 0.2% (w/v) BSA. |
| Storage | Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze. |
| Stability | Do not use after expiration date stamped on vial label. |
Safety Data Sheet
Technical Data Sheet
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