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CyFlow™ CD62L Alexa Fluor™ 700

CyFlow™ CD62L Alexa Fluor™ 700
Antigen: CD62L
Alternative Name: L-Selectin
Clone: DREG56
Application: Flow cytometry
Format/Fluorochrome: Alexa Fluor™ 700
Laser: Red
Target Species: Human
Field of Interest: Immunophenotyping
Species of Origin: Mouse
Regulatory Status: RUO
Clonality: monoclonal
Emission Maximum: 723 nm
Excitation Maximum: 702 nm
Isotype: IgG1
Order number: AU807163

For Research Use Only
Not for use in diagnostic or therapeutic procedures.

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EUROBIOTECH2017

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*1 Offer valid until 23/09/2017
Quantity 100 tests Volume 0.4 Immunogen PMA-activated human peripheral blood... more
CyFlow™ CD62L Alexa Fluor™ 700
Quantity100 tests
Volume0.4
ImmunogenPMA-activated human peripheral blood leukocytes
Background InformationCD62L (L-selectin) is an adhesion glycoprotein that is constitutively expressed on the cell surface of leukocytes and mediates their homing to inflammatory sites and peripheral lymph nodes by enabling rolling along the venular wall. CD62L is also involved in activation-induced neutrophil aggregation. Activation-dependent CD62L shedding, however, counteracts neutrophil rolling. CD62L has also signaling roles including enhance of chemokine receptor expression. Similarly to CD62P, the major ligand of CD62L is PSGL-1 (P-selectin glycoprotein ligand-1). The level of CD62L expression can be used to help distinguish naive T cells from effector/memory T cells.
UsageThe reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 4·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Storage BufferThe reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.

Specific References

| Kishimoto TK, Jutila MA, Butcher EC: Identification of a human peripheral lymph node homing receptor: a rapidly down‑regulated adhesion molecule. Proc·Natl·Acad·Sci·USA. 1990·Mar; 87(6):2244‑8. <·PMID:·2179952·> | Kishimoto TK, Warnock RA, Jutila MA, Butcher EC, Lane C, Anderson DC, Smith CW: Antibodies against human neutrophil LECAM‑1 (LAM‑1/Leu‑8/DREG‑56 antigen) and endothelial cell ELAM‑1 inhibit a common CD18‑independent adhesion pathway in vitro. Blood. 1991·Aug·1; 78(3):805‑11. <·PMID:·1713515·> | Doussis IA, Gatter KC, Mason DY: CD68 reactivity of non‑macrophage derived tumours in cytological specimens. J·Clin·Pathol. 1993·Apr; 46(4):334‑6. <·PMID:·7684403·> | Kishimoto T, Goyert S, Kikutani H, Mason D, Miyasaka M, Moretta L, Ohno T, Okumura K, Shaw S, Springer TA, Sugamura K, Sugawara H, von dem Borne AEGK, Zola H (Eds): Leucocyte Typing VI. Garland·Publishing·Inc,·New·York. 1997; 1‑1342. <·NLM·ID:·9712219·> | Abraham WM, Ahmed A, Sabater JR, Lauredo IT, Botvinnikova Y, Bjercke RJ, Hu X, Revelle BM, Kogan TP, Scott IL, Dixon RA, Yeh ET, Beck PJ: Selectin blockade prevents antigen‑induced late bronchial responses and airway hyperresponsiveness in allergic sheep. Am·J·Respir·Crit·Care·Med. 1999·Apr; 159(4‑1):1205‑14. <·PMID:·10194167·> | Jutila MA, Kurk S, Jackiw L, Knibbs RN, Stoolman LM: L‑selectin serves as an E‑selectin ligand on cultured human T lymphoblasts. J·Immunol. 2002·Aug·15; 169(4):1768‑73. <·PMID:·12165498·> | Xu T, Chen L, Shang X, Cui L, Luo J, Chen C, Ba X, Zeng X: Critical role of Lck in L‑selectin signaling induced by sulfatides engagement. J·Leukoc·Biol. 2008·Oct; 84(4):1192‑201. <·PMID:·18653462·> | Killock DJ, Parsons M, Zarrouk M, Ameer-Beg SM, Ridley AJ, Haskard DO, Zvelebil M, Ivetic A: In Vitro and in Vivo Characterization of Molecular Interactions between Calmodulin, Ezrin/Radixin/Moesin, and L‑selectin. J·Biol·Chem. 2009·Mar·27; 284(13):8833‑45. <·PMID:·19129194·> | Tu W, Mao H, Zheng J, Liu Y, Chiu SS, Qin G, Chan PL, Lam KT, Guan J, Zhang L, Guan Y, Yuen KY, Peiris JS, Lau YL: Cytotoxic T lymphocytes established by seasonal human influenza cross‑react against 2009 pandemic H1N1 influenza virus. J·Virol. 2010·Jul; 84(13):6527‑35. <·PMID:·20410263·>

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