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CyFlow™ CD62P APC

CyFlow™ CD62P APC
Antigen: CD62P
Alternative Name: P-selectin
Clone: AK4
Application: Flow cytometry
Format/Fluorochrome: APC
Laser: Red
Target Species: Human, Non-Human Primates
Field of Interest: Immunophenotyping
Species of Origin: Mouse
Regulatory Status: RUO
Clonality: monoclonal
Emission Maximum: 660 nm
Excitation Maximum: 650 nm
Isotype: IgG1
Order number: AV460497

For Research Use Only
Not for use in diagnostic or therapeutic procedures.

HLDA Workshop HLDA VI—WS Code P-44 Quantity 100 tests Volume 1 Immunogen Human... more
CyFlow™ CD62P APC
HLDA WorkshopHLDA VI—WS Code P-44
Quantity100 tests
Volume1
ImmunogenHuman platelets
Background InformationCD62P (P-selectin) is an adhesion glycoprotein that is expressed on platelets and endothelial cells upon their activation. Interaction between CD62P and its mucin-like ligand PSGL-1 (P-selectin glycoprotein ligand-1) expressed on the microvilli of most leukocytes supports leukocyte rolling along postkapillary venules at the earliest time of inflammation. Both CD62P and PSGL-1 are extended glycoproteins that form homodimers. CD62P dimerization is probably mediated through interactions of the transmembrane domains and stabilizes leukocyte tethering and rolling, probably by increasing rebinding within a bond cluster.
UsageThe reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 10·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
Storage BufferThe reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.

Specific References

| Dunlop LC, Skinner MP, Bendall LJ, Favaloro EJ, Castaldi PA, Gorman JJ, Gamble JR, Vadas MA, Berndt MC: Characterization of GMP‑140 (P‑selectin) as a circulating plasma protein. J·Exp·Med. 1992·Apr·1; 175(4):1147‑50. <·PMID:·1372646·> | Holme PA, Müller F, Solum NO, Brosstad F, Frøland SS, Aukrust P: Enhanced activation of platelets with abnormal release of RANTES in human immunodeficiency virus type 1 infection. FASEB·J. 1998·Jan; 12(1):79‑89. <·PMID:·9438413·> | Kowalska MA, Ratajczak J, Hoxie J, Brass LF, Gewirtz A, Poncz M, Ratajczak MZ: Megakaryocyte precursors, megakaryocytes and platelets express the HIV co‑receptor CXCR4 on their surface: determination of response to stromal‑derived factor‑1 by megakaryocytes and platelets. Br·J·Haematol. 1999·Feb; 104(2):220‑9. <·PMID:·10050701·> | Ludwig RJ, Schultz JE, Boehncke WH, Podda M, Tandi C, Krombach F, Baatz H, Kaufmann R, von Andrian UH, Zollner TM: Activated, not resting, platelets increase leukocyte rolling in murine skin utilizing a distinct set of adhesion molecules. J·Invest·Dermatol. 2004·Mar; 122(3):830‑6. <·PMID:·15086572·>