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CyFlow™ CD64 Low Endotoxin

CyFlow™ CD64 Low Endotoxin
Application: Flow cytometry, Functional assays, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western blot
Alternative Name: FcγRI
Antigen: CD64
Target Species: Human, Non-Human Primates
Format/Fluorochrome: Low Endotoxin
Clonality: monoclonal
Isotype: IgG1
Field of Interest: Immunophenotyping
Source Species: Mouse
Regulatory Status: RUO
Clone: 10.1
Order number: AR810750
HLDA Workshop HLDA III—WS Code M-250 Concentration Unit mg/mL Concentration 1... more
Product information "CyFlow™ CD64 Low Endotoxin"
HLDA WorkshopHLDA III—WS Code M-250
Concentration Unitmg/mL
Concentration1
Quantity0.1 mg
Volume0.1 mL
ImmunogenRheumatoid synovial fluid cells and fibronectin purified human monocytes
Background InformationCD64 (FcγRI) is a cell surface receptor for Fc region of IgG. It is composed of specific ligand binding α subunit and promiscuous γ subunit, which is indispensable for tyrosine-based signaling. However, even the α subunit can transduce signals leading to cellular effector functions. The isoform FcγRIa1 binds human IgG with high affinity, has limited myeloid cell distribution, and a relatively large intracellular domain. Products of related genes include FcγRIb and FcγRIc isoforms, but these specify low affinity IgG receptors if functionally expressed at all. Besides a role in antigen clearance, FcgammaRI (a1) can potently enhance MHC class I and II antigen presentation in vitro and in vivo.
Storage BufferThe reagent is provided in azide-free phosphate buffered saline (PBS) solution, pH ≈7.4; 0.2 µm filter sterilized. Endotoxin level is less than 0.01 EU/µg of the protein, as determined by the LAL test.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.
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