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CyFlow™ CD71 FITC

CyFlow™ CD71 FITC
Application: Flow cytometry
Field of Interest: Endocytosis, Immunophenotyping
Alternative Name: T9, TFR, TRFR
Antigen: CD71
Isotype: IgG1
Source Species: Mouse
Clonality: monoclonal
Emission Maximum: 518 nm
Excitation Maximum: 490 to 495 nm
Laser: Blue
Regulatory Status: RUO
Target Species: Human
Format/Fluorochrome: FITC
Clone: MEM-75
Order number: AR351095
HLDA Workshop HLDA IV—WS Code A 045 HLDA V—WS Code T T-165 Quantity 100 tests Volume... more
Product information "CyFlow™ CD71 FITC"
HLDA WorkshopHLDA IV—WS Code A 045 HLDA V—WS Code T T-165
Quantity100 tests
Volume2.0 mL
ImmunogenNALM-6 human pre-B cell line
Background InformationCD71 (transferrin receptor) is a type II transmembrane glycoprotein expressed as homodimer in erythroid blood cell line and in activated leukocytes. Upon binding of holotransferrin (complex of transferrin and iron ions), CD71 is internalized by clathrin-mediated endocytosis. Acidification of endosomes by vesicular membrane proton pumps leads to dissociation of iron ions, whereas transferrin (apotransferrin) remains associated with CD71 and recycles to the cell surface, where it is released upon exposure to normal pH. CD71 is also involved in uptake of non-transferrin bound iron.
UsageThe reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 20·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.
Storage BufferThe reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide and 0.2% (w/v) BSA.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.
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