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CyFlow™ IgG2a Alexa Fluor™ 647
| Clonality: | monoclonal |
| Regulatory Status: | RUO |
| Source Species: | Mouse |
| Laser: | Red |
| Excitation Maximum: | 650 nm |
| Isotype: | IgG2a |
| Format/Fluorochrome: | Alexa Fluor™ 647 |
| Emission Maximum: | 665 nm |
| Application: | Flow cytometry (Isotype control) |
| Field of Interest: | Isotype controls |
| Antigen: | IgG2a Isotype Control |
| Clone: | MOPC-173 |
| Order number: | AL914834 |
For Research Use Only
Not for use in diagnostic or therapeutic procedures.
Concentration Unit mg/mL Concentration 0,1 Quantity 0.1 mg Volume 1.0 mL... more
Product information "CyFlow™ IgG2a Alexa Fluor™ 647"
| Concentration Unit | mg/mL |
| Concentration | 0,1 |
| Quantity | 0.1 mg |
| Volume | 1.0 mL |
| Immunogen | The transplantable plasmacytoma MOPC-173 was induced by intraperitoneal injection of mineral oils into BALB/c mice |
| Background Information | The specificity of staining by monoclonal antibodies to target antigens should be verified by establishing the amount of non-specific antibody binding. Especially at higher concentration (more than 15 μ/ml) the antibody staining usually has consignable background. To this end a non-reactive immunoglobulin of the same isotype is included as a negative control for each specific monoclonal antibody used in a particular immunoassay. The monoclonal antibody MOPC-173, generated against an undefined antigen, does not react specifically with mouse, rat and human samples, and hence all the background that could be observed when working with this antibody would be a result of general nonspecific interactions between an mouse IgG2a molecule and the respective sample under the particular conditions. This shall help the customer to set up the experimental conditions so that the nonspecific binding of any antibody is abolished. |
| Usage | The reagent is designed as an isotype control for Flow Cytometry analysis. To establish the amount of non-specific antibody binding, match the concentration of the correct isotype to the recommended working concentration of the antigen-specific antibody. If the background signal of the isotype control is too high (usually when working antibody concentrations are above 10·µg/ml of incubation mixture), change the experimental conditions to reduce the background. |
| Storage Buffer | The reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide. |
| Storage | Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze. |
| Stability | Do not use after expiration date stamped on vial label. |
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