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CyFlow™ CD22 PE-Cy7

CyFlow™ CD22 PE-Cy7
Application: Flow cytometry
Isotype: IgG1
Alternative Name: BL-CAM, SIGLEC-2, SIGLEC2
Antigen: CD22
Emission Maximum: 785 nm
Format/Fluorochrome: PE-Cy7
Excitation Maximum: 496 nm, 565 nm
Clonality: monoclonal
Laser: Blue, Green, Yellow
Field of Interest: Immunophenotyping
Source Species: Mouse
Regulatory Status: RUO
Target Species: Human
Clone: IS7
Order number: CT499497
Quantity 100 tests Volume 0.4 mL Immunogen Reh human cell line Background... more
Product information "CyFlow™ CD22 PE-Cy7"
Quantity100 tests
Volume0.4 mL
ImmunogenReh human cell line
Background InformationCD22 (Siglec-2; sialic acid-binding immunoglobulin-like lectin-2) is a transmembrane glycoprotein binding α 2,6-linked sialic acid-bearing ligands. Intracellular domain of CD22 recruits protein tyrosine phosphatase SHP-1 through the immunoreceptor tyrosine-based inhibitory motifs (ITIMs), thus setting a treshold for B cell receptor-mediated activation. CD22 also regulates B-cell response by involvement in controlling the CD19/CD21-Src-family protein tyrosine kinase amplification pathway and CD40 signaling. CD22 exhibits hallmarks of clathrin-mediated endocytic pathway.
UsageThe reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 4·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Storage BufferThe reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide and 0.2% (w/v) BSA.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.
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