Welcome to
Sysmex Flow Cytometry Other European Country!

For ordering please make sure you are in your local shop.
If you are from Other European Country then please continue.
Otherwise please go to your country.

Go to my country Continue

Article successfully added.

CyFlow™ CD22 PE

CyFlow™ CD22 PE
Antigen: CD22
Alternative Name: BL-CAM, SIGLEC-2, SIGLEC2
Clone: S-HCL-1
Application: Flow cytometry
Format/Fluorochrome: PE
Laser: Blue, Green, Yellow
Target Species: Human
Field of Interest: Immunophenotyping
Species of Origin: Mouse
Regulatory Status: RUO
Clonality: monoclonal
Emission Maximum: 576 nm
Excitation Maximum: 496 nm, 565 nm
Isotype: IgG2b
Order number: CS494603

For Research Use Only
Not for use in diagnostic or therapeutic procedures.

HLDA Workshop HLDA IV—WS Code B48 Quantity 100 tests Volume 1 Immunogen Whole... more
CyFlow™ CD22 PE
HLDA WorkshopHLDA IV—WS Code B48
Quantity100 tests
ImmunogenWhole hairy cell leukemia cells and membrane preparation
Background InformationCD22 (Siglec-2; sialic acid-binding immunoglobulin-like lectin-2) is a transmembrane glycoprotein binding α 2,6-linked sialic acid-bearing ligands. Intracellular domain of CD22 recruits protein tyrosine phosphatase SHP-1 through the immunoreceptor tyrosine-based inhibitory motifs (ITIMs), thus setting a treshold for B cell receptor-mediated activation. CD22 also regulates B-cell response by involvement in controlling the CD19/CD21-Src-family protein tyrosine kinase amplification pathway and CD40 signaling. CD22 exhibits hallmarks of clathrin-mediated endocytic pathway.
UsageThe reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 10·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
Storage BufferThe reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.
Specific References

| Foon KA, Todd RF rd: Immunologic classification of leukemia and lymphoma. Blood. 1986·Jul; 68(1):11324. <·PMID:·2809561·> | Huang J, Fan G, Zhong Y, Gatter K, Braziel R, Gross G, Bakke A: Diagnostic usefulness of aberrant CD22 expression in differentiating neoplastic cells of B‑Cell chronic lymphoproliferative disorders from admixed benign B cells in four‑color multiparameter flow cytometry. Am·J·Clin·Pathol. 2005·Jun; 123(6):826‑32. <·PMID:·15899772·> | James SE, Greenberg PD, Jensen MC, Lin Y, Wang J, Till BG, Raubitschek AA, Forman SJ, Press OW: Antigen sensitivity of CD22‑specific chimeric TCR is modulated by target epitope distance from the cell membrane. J·Immunol. 2008·May·15; 180(10):7028‑38. <·PMID:·18453625·> | Reineks EZ, Osei ES, Rosenberg A, Auletta J, Meyerson HJ: CD22 expression on blastic plasmacytoid dendritic cell neoplasms and reactivity of anti‑CD22 antibodies to peripheral blood dendritic cells. Cytometry·B·Clin·Cytom. 2009·Jul; 76(4):237‑48. <·PMID:·19382197·>