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CyFlow™ CD22 PE-Cy7
Application: | Flow cytometry |
Excitation Maximum: | 496 nm, 565 nm |
Alternative Name: | BL-CAM, SIGLEC-2, SIGLEC2 |
Antigen: | CD22 |
Emission Maximum: | 785 nm |
Format/Fluorochrome: | PE-Cy7 |
Laser: | Blue, Green, Yellow |
Clonality: | monoclonal |
Field of Interest: | Immunophenotyping |
Source Species: | Mouse |
Isotype: | IgG2b |
Regulatory Status: | RUO |
Target Species: | Human |
Clone: | S-HCL-1 |
Order number: | CN265686 |
For Research Use Only
Not for use in diagnostic or therapeutic procedures.
HLDA Workshop HLDA IV—WS Code B48 Quantity 100 tests Volume 0.4 mL Immunogen... more
Product information "CyFlow™ CD22 PE-Cy7"
HLDA Workshop | HLDA IV—WS Code B48 |
Quantity | 100 tests |
Volume | 0.4 mL |
Immunogen | Whole hairy cell leukemia cells and membrane preparation |
Background Information | CD22 (Siglec-2; sialic acid-binding immunoglobulin-like lectin-2) is a transmembrane glycoprotein binding α 2,6-linked sialic acid-bearing ligands. Intracellular domain of CD22 recruits protein tyrosine phosphatase SHP-1 through the immunoreceptor tyrosine-based inhibitory motifs (ITIMs), thus setting a treshold for B cell receptor-mediated activation. CD22 also regulates B-cell response by involvement in controlling the CD19/CD21-Src-family protein tyrosine kinase amplification pathway and CD40 signaling. CD22 exhibits hallmarks of clathrin-mediated endocytic pathway. |
Usage | The reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 4·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests. |
Storage Buffer | The reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide and 0.2% (w/v) BSA. |
Storage | Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze. |
Stability | Do not use after expiration date stamped on vial label. |
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