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CyFlow™ CD43 FITC

CyFlow™ CD43 FITC
Application: Flow cytometry
Clonality: monoclonal
Format/Fluorochrome: FITC
Target Species: Human
Regulatory Status: RUO
Laser: Blue
Excitation Maximum: 490 to 495 nm
Emission Maximum: 518 nm
Source Species: Mouse
Field of Interest: Immunophenotyping
Isotype: IgG1
Antigen: CD43
Alternative Name: Leukosialin, sialophorin
Clone: MEM-59
Order number: BR081923
HLDA Workshop HLDA IV—WS Code N 604 HLDA V—WS Code AS S290 Quantity 100 tests Volume... more
Product information "CyFlow™ CD43 FITC"
HLDA WorkshopHLDA IV—WS Code N 604 HLDA V—WS Code AS S290
Quantity100 tests
Volume2.0 mL
ImmunogenHuman T lymphocytes
Background InformationCD43 (leukosialin, sialophorin) is a transmembrane mucin-like protein with high negative charge, expressed on the surface of most hematopoietic cells. CD43 contributes to a repulsive barrier that interferes with cellular adhesion, however, in certain cases also promotes leukocyte aggregation. By interaction with actin-binding proteins ezrin and moesin, CD43 plays a regulatory role in remodeling T-cell morphology and regulates cell-cell interactions during lymphocyte traffic. CD43 signaling both enhances LFA-1 adhesiveness and counteracts LFA-1 induction via other receptors. Expression of CD43 causes induction of functionally active tumor suppressor p53 protein, but in case of p53 and ARF defficiency CD43 promotes tumour proliferation and viability. It appears to be an important modulator of leukocyte functions.
UsageThe reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 20·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.
Storage BufferThe reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide and 0.2% (w/v) BSA.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.
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