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CyFlow™ CD43 Low Endotoxin

CyFlow™ CD43 Low Endotoxin
Application: Flow cytometry, Functional assays, Immunohistochmistry (paraffin-embedded sections), Immunoprecipitation, Western blot
Clonality: monoclonal
Target Species: Human
Regulatory Status: RUO
Source Species: Mouse
Field of Interest: Immunophenotyping
Isotype: IgG1
Format/Fluorochrome: Low Endotoxin
Antigen: CD43
Alternative Name: Leukosialin, sialophorin
Clone: MEM-59
Order number: CU046978
HLDA Workshop HLDA IV—WS Code N 604 HLDA V—WS Code AS S290 Concentration Unit mg/mL... more
Product information "CyFlow™ CD43 Low Endotoxin"
HLDA WorkshopHLDA IV—WS Code N 604 HLDA V—WS Code AS S290
Concentration Unitmg/mL
Concentration1
Quantity0.1 mg
Volume0.1 mL
ImmunogenHuman T lymphocytes
Background InformationCD43 (leukosialin, sialophorin) is a transmembrane mucin-like protein with high negative charge, expressed on the surface of most hematopoietic cells. CD43 contributes to a repulsive barrier that interferes with cellular adhesion, however, in certain cases also promotes leukocyte aggregation. By interaction with actin-binding proteins ezrin and moesin, CD43 plays a regulatory role in remodeling T-cell morphology and regulates cell-cell interactions during lymphocyte traffic. CD43 signaling both enhances LFA-1 adhesiveness and counteracts LFA-1 induction via other receptors. Expression of CD43 causes induction of functionally active tumor suppressor p53 protein, but in case of p53 and ARF defficiency CD43 promotes tumour proliferation and viability. It appears to be an important modulator of leukocyte functions.
Storage BufferThe reagent is provided in azide-free phosphate buffered saline (PBS) solution, pH ≈7.4; 0.2 µm filter sterilized. Endotoxin level is less than 0.01 EU/µg of the protein, as determined by the LAL test.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.
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