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CyFlow™ CD45 Pacific Blue™
Application: | Flow cytometry |
Excitation Maximum: | 400 to 410 nm |
Clone: | MEM-28 |
Alternative Name: | B220, LCA, T200 |
Antigen: | CD45 |
Emission Maximum: | 455 nm |
Laser: | Violet |
Clonality: | monoclonal |
Format/Fluorochrome: | Pacific Blue™ |
Isotype: | IgG1 |
Field of Interest: | Immunophenotyping, Phosphorylation |
Source Species: | Mouse |
Regulatory Status: | RUO |
Target Species: | Human |
Order number: | BK321039 |
For Research Use Only
Not for use in diagnostic or therapeutic procedures.
Quantity 100 tests Volume 0.4 mL Immunogen Human thymocytes and T lymphocytes... more
Product information "CyFlow™ CD45 Pacific Blue™"
Quantity | 100 tests |
Volume | 0.4 mL |
Immunogen | Human thymocytes and T lymphocytes |
Background Information | CD45 (LCA; leukocyte common antigen) is a receptor-type protein tyrosine phosphatase ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms as a result of alternative splicing; these isoforms differ in their extracellular domains, whereas they share identical transmembrane and cytoplasmic domains. These isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. Besides the role in immunoreceptor signaling, CD45 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. |
Usage | The reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 4·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests. |
Storage Buffer | The reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide and 0.2% (w/v) BSA. |
Storage | Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze. |
Stability | Do not use after expiration date stamped on vial label. |
Safety Data Sheet
Technical Data Sheet
- CyFlow™ CD45 Pacific Blue™ (2D1) AJ846005 TDS US,CA EN.pdf
- CyFlow CD45 Pacific Blue (MEM-28) BK321039 TDS RUO.pdf
- CyFlow CD45 Pacific Blue (2D1) AJ846005 TDS ASR EN.pdf
Instruction for Use
- CyFlow CD45 Pacific Blue (2D1) AS153983 IFU CZ CZ.pdf
- CyFlow CD45 Pacific Blue (2D1) AS153983 IFU DK DK.pdf
- CyFlow CD45 Pacific Blue (2D1) AS153983 IFU GR EL.pdf
- CyFlow CD45 Pacific Blue (2D1) AS153983 IFU IT IT.pdf
- CyFlow CD45 Pacific Blue (2D1) AS153983 IFU NL NL.pdf
- CyFlow CD45 Pacific Blue (2D1) AS153983 IFU NO NO.pdf
- CyFlow CD45 Pacific Blue (2D1) AS153983 IFU PL PL.pdf
- CyFlow CD45 Pacific Blue (2D1) AS153983 IFU RU RU.pdf
- CyFlow CD45 Pacific Blue (2D1) AS153983 IFU SE SV.pdf
- CyFlow CD45 Pacific Blue (2D1) AS153983 IFU SK SK.pdf
- CyFlow CD45 Pacific Blue (2D1) AS153983 IFU TR TR.pdf
- CyFlow CD45 Pacific Blue (2D1) AU857794 IFU CZ CZ.pdf
- CyFlow CD45 Pacific Blue (2D1) AS153983 IFU DE DE.pdf
- CyFlow CD45 Pacific Blue (2D1) AS153983 IFU ES ES.pdf
- CyFlow CD45 Pacific Blue (2D1) AS153983 IFU FR FR.pdf
- CyFlow CD45 Pacific Blue (2D1) AS153983 IFU GB EN.pdf
- CyFlow CD45 Pacific Blue (2D1) AS153983 IFU PT PT.pdf
- CyFlow CD45 Pacific Blue (2D1) AU857794 IFU DE DE.pdf
- CyFlow CD45 Pacific Blue (2D1) AU857794 IFU DK DK.pdf
- CyFlow CD45 Pacific Blue (2D1) AU857794 IFU ES ES.pdf
- CyFlow CD45 Pacific Blue (2D1) AU857794 IFU FR FR.pdf
- CyFlow CD45 Pacific Blue (2D1) AU857794 IFU GB EN.pdf
- CyFlow CD45 Pacific Blue (2D1) AU857794 IFU GR EL.pdf
- CyFlow CD45 Pacific Blue (2D1) AU857794 IFU IT IT.pdf
- CyFlow CD45 Pacific Blue (2D1) AU857794 IFU NL NL.pdf
- CyFlow CD45 Pacific Blue (2D1) AU857794 IFU BG BG.pdf
- CyFlow CD45 Pacific Blue (2D1) AU857794 IFU HR HR.pdf
- CyFlow CD45 Pacific Blue (2D1) AU857794 IFU RO RO.pdf
- CyFlow CD45 Pacific Blue (2D1) AU857794 IFU SI SL.pdf
- CyFlow CD45 Pacific Blue (2D1) AU857794 IFU RS SR.pdf
ECDoC
- CyFlow CD45 Pacific Blue AS153983 ECDOC EN-DE.pdf
- CyFlow CD45 Pacific Blue AU857794 ECDOC EN-DE.pdf
- CyFlow CD45 Pacific Blue AU857794 ECDOC EN-DE..pdf
Certificate Of Analysis
- CyFlow™ CD45 Pacific Blue™_AJ846005_COA_901307.pdf
- CyFlow™ CD45 Pacific Blue™_AS153983_COA_901307.pdf
- CyFlow CD45 Pacific Blue AJ846005 COA 902167.pdf
- CyFlow CD45 Pacific Blue AS153983 COA 902167.pdf
- CyFlow CD45 Pacific Blue AJ846005 COA 904057.pdf
- CyFlow CD45 Pacific Blue AU857794 COA 905140.pdf
- CyFlow CD45 Pacific Blue AJ846005 COA 905140.pdf
- CyFlow CD45 Pacific Blue AJ846005 COA 907107.pdf
- CyFlow CD45 Pacific Blue AJ846005 COA 907987.pdf
- CyFlow CD45 Pacific Blue AJ846005 COA 908366.pdf
- CyFlow CD45 Pacific Blue AU857794 COA 909028.pdf
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