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CyFlow™ CD45 FITC
| Application: | Flow cytometry |
| Field of Interest: | Immunophenotyping, Phosphorylation |
| Clone: | MEM-28 |
| Alternative Name: | B220, LCA, T200 |
| Antigen: | CD45 |
| Isotype: | IgG1 |
| Source Species: | Mouse |
| Clonality: | monoclonal |
| Emission Maximum: | 518 nm |
| Excitation Maximum: | 490 to 495 nm |
| Laser: | Blue |
| Regulatory Status: | RUO |
| Target Species: | Human |
| Format/Fluorochrome: | FITC |
| Order number: | AQ194821 |
For Research Use Only
Not for use in diagnostic or therapeutic procedures.
Quantity 100 tests Volume 2.0 mL Immunogen Human thymocytes and T lymphocytes... more
Product information "CyFlow™ CD45 FITC"
| Quantity | 100 tests |
| Volume | 2.0 mL |
| Immunogen | Human thymocytes and T lymphocytes |
| Background Information | CD45 (LCA; leukocyte common antigen) is a receptor-type protein tyrosine phosphatase ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms as a result of alternative splicing; these isoforms differ in their extracellular domains, whereas they share identical transmembrane and cytoplasmic domains. These isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. Besides the role in immunoreceptor signaling, CD45 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. |
| Usage | The reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 20·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests. |
| Storage Buffer | The reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide and 0.2% (w/v) BSA. |
| Storage | Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze. |
| Stability | Do not use after expiration date stamped on vial label. |
Safety Data Sheet
Technical Data Sheet
- CyFlow™ CD45 FITC (2D1) AG109229 TDS US,CA EN.pdf
- CyFlow CD45 FITC (MEM-28) AQ194821 TDS RUO.pdf
- CyFlow CD45 FITC (2D1) AG109229 TDS ASR EN.pdf
Instruction for Use
- CyFlow CD45 FITC (2D1) AW518513 IFU CZ CZ.pdf
- CyFlow CD45 FITC (2D1) BJ120977 IFU CZ CZ.pdf
- CyFlow CD45 FITC (2D1) BJ120977 IFU DK DK.pdf
- CyFlow CD45 FITC (2D1) BJ120977 IFU GR EL.pdf
- CyFlow CD45 FITC (2D1) BJ120977 IFU IT IT.pdf
- CyFlow CD45 FITC (2D1) BJ120977 IFU NL NL.pdf
- CyFlow CD45 FITC (2D1) BJ120977 IFU NO NO.pdf
- CyFlow CD45 FITC (2D1) BJ120977 IFU PL PL.pdf
- CyFlow CD45 FITC (2D1) BJ120977 IFU RU RU.pdf
- CyFlow CD45 FITC (2D1) BJ120977 IFU SE SV.pdf
- CyFlow CD45 FITC (2D1) BJ120977 IFU SK SK.pdf
- CyFlow CD45 FITC (2D1) BJ120977 IFU TR TR.pdf
- CyFlow CD45 FITC (2D1) BJ120977 IFU DE DE.pdf
- CyFlow CD45 FITC (2D1) AW518513 IFU DE DE.pdf
- CyFlow CD45 FITC (2D1) AW518513 IFU DK DK.pdf
- CyFlow CD45 FITC (2D1) AW518513 IFU ES ES.pdf
- CyFlow CD45 FITC (2D1) AW518513 IFU FR FR.pdf
- CyFlow CD45 FITC (2D1) AW518513 IFU GB EN.pdf
- CyFlow CD45 FITC (2D1) AW518513 IFU GR EL.pdf
- CyFlow CD45 FITC (2D1) AW518513 IFU IT IT.pdf
- CyFlow CD45 FITC (2D1) AW518513 IFU NL NL.pdf
- CyFlow CD45 FITC (2D1) AW518513 IFU NO NO.pdf
- CyFlow CD45 FITC (2D1) AW518513 IFU PL PL.pdf
- CyFlow CD45 FITC (2D1) AW518513 IFU PT PT.pdf
- CyFlow CD45 FITC (2D1) AW518513 IFU RU RU.pdf
- CyFlow CD45 FITC (2D1) AW518513 IFU SE SV.pdf
- CyFlow CD45 FITC (2D1) AW518513 IFU SK SK.pdf
- CyFlow CD45 FITC (2D1) AW518513 IFU TR TR.pdf
- CyFlow CD45 FITC (2D1) BJ120977 IFU ES ES.pdf
- CyFlow CD45 FITC (2D1) BJ120977 IFU FR FR.pdf
- CyFlow CD45 FITC (2D1) BJ120977 IFU GB EN.pdf
- CyFlow CD45 FITC (2D1) BJ120977 IFU PT PT.pdf
- CyFlow CD45 FITC (2D1) AW518513 IFU BG BG.pdf
- CyFlow CD45 FITC (2D1) AW518513 IFU HR HR.pdf
- CyFlow CD45 FITC (2D1) AW518513 IFU RO RO.pdf
- CyFlow CD45 FITC (2D1) AW518513 IFU SI SL.pdf
- CyFlow CD45 FITC (2D1) AW518513 IFU RS SR.pdf
ECDoC
- CyFlow CD45 FITC AW518513 ECDOC EN-DE.pdf
- CyFlow CD45 FITC BJ120977 ECDOC EN-DE.pdf
- CyFlow CD45 FITC AW518513 ECDOC EN-DE..pdf
Certificate Of Analysis
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