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CyFlow™ CD59 PE

CyFlow™ CD59 PE
Antigen: CD59
Alternative Name: Protectin H19
Clone: MEM-43
Application: Flow cytometry
Format/Fluorochrome: PE
Laser: Blue, Green, Yellow
Target Species: Human
Field of Interest: Immunophenotyping
Species of Origin: Mouse
Regulatory Status: RUO
Clonality: monoclonal
Emission Maximum: 576 nm
Excitation Maximum: 496 nm, 565 nm
Isotype: IgG2a
Order number: AU678455

For Research Use Only
Not for use in diagnostic or therapeutic procedures.

Quantity 100 tests Volume 2 Immunogen Thymocytes and T lymphocytes Background... more
CyFlow™ CD59 PE
Quantity100 tests
ImmunogenThymocytes and T lymphocytes
Background InformationCD59 (Protectin) is a small (18-20 kDa) GPI-anchored ubiquitously expressed inhibitor of the membrane attack complex (MAC). It is thus the key regulator that preserves the autologous cells from terminal effector mechanism of the complement cascade. CD59 associates with C5b-8 complex and thereby counteracts appropriate formation of cytolytic pore within the plasma membrane. CD59 is also an low-affinity ligand of human CD2 and causes T cell costimulation.
UsageThe reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 20·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.
Storage BufferThe reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.
Specific References

| Horejsi V, Hilgert I, Kristofova H, Bazil V, Bukovsky A, Kulhankova J: Monoclonal antibodies against human leucocyte antigens (I); Antibodies against beta‑2‑microglobulin, immunoglobulin kappa light chains, HLA‑DR‑like antigens, T8 antigen, T1 antigen, a monocyte antigen, and a pan‑leucocyte antigen. Folia·Biol·(Praha). 1986; 32(1):12‑25. <·PMID:·2422063·> | Forsberg UH, Bazil V, Stefanova I, Schroder J: Gene for human CD59 (likely Ly‑6 homologue) is located on the short arm of chromosome 11. Immunogenetics. 1989; 30(3):188‑93. <·PMID:·2476389·> | Knapp W, Dorken B, Gilks W, Rieber EP, Schmidt RE, Stein H, von dem Borne AEGK (Eds): Leucocyte Typing IV. Oxford·University·Press,·Oxford. 1989; 1‑1820. <·NLM·ID:·8914679·> | Stefanova I, Hilgert I, Kristofova H, Brown R, Low MG, Horejsi V: Characterization of a broadly expressed human leucocyte surface antigen MEM‑43 anchored in membrane through phosphatidylinositol. Mol·Immunol. 1989·Feb; 26(2):153‑61. <·PMID:·2918859·> | Stefanova I, Horejsi V, Ansotegui IJ, Knapp W, Stockinger H: GPI‑anchored cell‑surface molecules complexed to protein tyrosine kinases. Science. 1991·Nov·15; 254(5034):1016‑9. <·PMID:·1719635·> | Cinek T, Horejsi V: The nature of large noncovalent complexes containing glycosyl‑phosphatidylinositol‑anchored membrane glycoproteins and protein tyrosine kinases.. J·Immunol. 1992·Oct·1; 149(7):2262‑70. <·PMID:·1382093·> | Doussis IA, Gatter KC, Mason DY: CD68 reactivity of non‑macrophage derived tumours in cytological specimens. J·Clin·Pathol. 1993·Apr; 46(4):334‑6. <·PMID:·7684403·> | Bodian DL, Davis SJ, Morgan BP, Rushmere NK: Mutational analysis of the active site and antibody epitopes of the complement‑inhibitory glycoprotein, CD59. J·Exp·Med. 1997·Feb·3; 185(3):507‑16. <·PMID:·9053451·> | Cebecauer M, Cerny J, Horejsi V: Incorporation of leucocyte GPI‑anchored proteins and protein tyrosine kinases into lipid‑rich membrane domains of COS‑7 cells. Biochem·Biophys·Res·Commun. 1998·Feb·24; 243(3):706‑10. <·PMID:·9500981·> | Menier C, Saez B, Horejsi V, Martinozzi S, Krawice-Radanne I, Bruel S, Le Danff C, Reboul M, Hilgert I, Rabreau M, Larrad ML, Pla M, Carosella ED, Rouas-Freiss N: Characterization of monoclonal antibodies recognizing HLA‑G or HLA‑E: new tools to analyze the expression of nonclassical HLA class I molecules. Hum·Immunol. 2003·Mar; 64(3):315‑26. <·PMID:·12590976·> | Romero X, Zapater N, Calvo M, Kalko SG, de la Fuente MA, Tovar V, Ockeloen C, Pizcueta P, Engel P: CD229 (Ly{09} lymphocyte cell surface receptor interacts homophilically through its N‑terminal domain and relocalizes to the immunological synapse. J·Immunol. 2005·Jun·1; 174(11):7033‑42. <·PMID:·15905546·> | Omidvar N, Wang EC, Brennan P, Longhi MP, Smith RA, Morgan BP: Expression of glycosylphosphatidylinositol‑anchored CD59 on target cells enhances human NK cell‑mediated cytotoxicity. J·Immunol. 2006·Mar·1; 176(5):2915‑23. <·PMID:·16493049·> | Meraner P, Horejsí V, Wolpl A, Fischer GF, Stingl G, Maurer D: Dendritic cells sensitize TCRs through self‑MHC‑mediated Src family kinase activation. J·Immunol. 2007·Feb·15; 178(4):2262‑71. <·PMID:·17277131·> | Ide K, Wang H, Tahara H, Liu J, Wang X, Asahara T, Sykes M, Yang YG, Ohdan H: Role for CD47‑SIRPalpha signaling in xenograft rejection by macrophages. Proc·Natl·Acad·Sci·USA. 2007·Mar·20; 104(12):5062‑6. <·PMID:·17360380·> | Awasthi S, Wolf R, White G: Ontogeny and phagocytic function of baboon lung dendritic cells. Immunol·Cell·Biol. 2009·Jul; 87(5):419‑27. <·PMID:·19223853·> | Sutherland DR, Keeney M, Illingworth A: Practical guidelines for the high‑sensitivity detection and monitoring of paroxysmal nocturnal hemoglobinuria (PNH) clones by flow cytometry. Cytometry·B·Clin·Cytom. 2012·Jul; 82(4):195‑208. <·PMID:·22499484·>