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CyFlow™ ARHGEF4 Purified
| Antigen: | ARHGEF4 |
| Clone: | ARHGEF-08 |
| Application: | Flow cytometry, Western blot |
| Format/Fluorochrome: | Purified |
| Target Species: | Human |
| Field of Interest: | Signaling |
| Species of Origin: | Mouse |
| Regulatory Status: | RUO |
| Clonality: | monoclonal |
| Isotype: | IgG1 |
| Concentration Unit | mg/mL |
| Concentration | 1 |
| Quantity | 0.1 mg |
| Volume | 0.1 |
| Immunogen | Recombinant fragment of human ARHGEF4 (amino acids 143-271) |
| Background Information | ARHGEF4 (Rho guanine nucleotide exchange factor 4), also known as ASEF1 (Adenomatous polyposis coli - stimulated guanine nucleotide exchange factor 1) is an approximately 80 kDa cytoplasmic protein important for growth factor-mediated regulation of cell morphology and migration. Besides N-terminal adenomatous polyposis coli (APC)-binding region (ABR) it contains Dbl homology (DH), Pleckstrin homology (PH) and SH3 domains. The SH3 domain inhibits GEF activity of ARHGEF4 by intramolecular interaction with the DH domain, whereas binding of APC stimulates the GEF activity. Activated ARHGEF4 stimulates the small GTPase Cdc42, which leads to decreased cell-cell adherence and enhanced cell migration. |
| Storage Buffer | The reagent is provided in phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide. |
| Storage | Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze. |
| Stability | Do not use after expiration date stamped on vial label. |
Specific References
| Kawasaki Y, Tsuji S, Sagara M, Echizen K, Shibata Y, Akiyama T: Adenomatous polyposis coli and Asef function downstream of hepatocyte growth factor and phosphatidylinositol 3‑kinase. J·Biol·Chem. 2009·Aug·14; 284(33):22436‑43. <·PMID:·19525225·> | Lyons R, Williams O, Morrow M, Sebire N, Hubank M, Anderson J: The RAC specific guanine nucleotide exchange factor Asef functions downstream from TEL‑AML1 to promote leukaemic transformation. Leuk·Res. 2010·Jan; 34(1):109‑15. <·PMID:·19628279·> | Zhang Z, Chen L, Gao L, Lin K, Zhu L, Lu Y, Shi X, Gao Y, Zhou J, Xu P, Zhang J, Wu G: Structural basis for the recognition of Asef by adenomatous polyposis coli. Cell·Res. 2012·Feb; 22(2):372‑86. <·PMID:·21788986·>
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