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CyFlow™ Ki-67 Alexa Fluor™ 488

CyFlow™ Ki-67 Alexa Fluor™ 488
Application: Flow cytometry
Clonality: monoclonal
Target Species: Cow, Human
Regulatory Status: RUO
Laser: Blue
Source Species: Mouse
Isotype: IgG1
Format/Fluorochrome: Alexa Fluor™ 488
Excitation Maximum: 495 nm
Emission Maximum: 519 nm
Field of Interest: Transcription
Antigen: Ki-67
Clone: Ki-67
Order number: BN097991
Quantity 100 tests Volume 0.4 mL Immunogen Nuclei of the Hodgkin lymphoma cell line... more
Product information "CyFlow™ Ki-67 Alexa Fluor™ 488"
Quantity100 tests
Volume0.4 mL
ImmunogenNuclei of the Hodgkin lymphoma cell line L428
Background InformationKi-67 is a highly protease-sensitive nuclear protein expressed in two isoforms (345 kDa and 395 kDa), both of which are identified by the antibody clone Ki-67. The Ki-67 antigen is essential for cell proliferation and its expression is restricted to the cycling cells. It is detected in G1, S, G2 and M phase, whereas it is absent in cells which are in G0 phase and it is not associated with DNA repair processes. Ki-67 thus represents an important tool for detection of proliferating cells, which is of great importance in tumor diagnostics and is commonly used as a prognostic factor in cancer studies.
UsageThe reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 4·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Storage BufferThe reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide and 0.2% (w/v) BSA.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.
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