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CyFlow™ TNF-alpha APC

CyFlow™ TNF-alpha APC
Application: Flow cytometry
Clonality: monoclonal
Target Species: Human, Non-Human Primates, Pig
Regulatory Status: RUO
Source Species: Mouse
Format/Fluorochrome: APC
Laser: Red
Excitation Maximum: 650 nm
Emission Maximum: 660 nm
Isotype: IgG1
Field of Interest: Signaling
Antigen: TNF-α
Clone: MAb11
Order number: AF800796
Quantity 100 tests Volume 1.0 mL Immunogen Recombinant human TNF α Background... more
Product information "CyFlow™ TNF-alpha APC"
Quantity100 tests
Volume1.0 mL
ImmunogenRecombinant human TNF α
Background InformationTNF α is a cytokine produced by monocytes, macrophages, neutrophils, NK cells, CD4+ T cells and many transformed cells. It can be expressed as a 17 kDa free molecule, or as a 26 kDa membrane protein. TNF α easily forms stable trimers, but also other multimeric complexes. In the immune system, it is an important regulator, which has cytolytic and cytostatic activity against a range of tumor cells, increases fibroblast proliferation and supports neutrophil chemotaxis and phagocytosis.
UsageThe reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 10·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
Storage BufferThe reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide and 0.2% (w/v) BSA.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.
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