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CyFlow™ IgG2a PE

CyFlow™ IgG2a PE
Species of Origin: Mouse
Antigen: IgG2a Isotype Control
Clone: PPV-04
Regulatory Status: RUO
Application: Flow cytometry (Isotype control)
Format/Fluorochrome: PE
Laser: Blue, Green, Yellow
Field of Interest: Isotype controls
Clonality: monoclonal
Emission Maximum: 576 nm
Excitation Maximum: 496 nm, 565 nm
Isotype: IgG2a
Order number: CV070533
The specificity of staining by monoclonal antibodies to target antigens should be verified by... more
Product information "CyFlow™ IgG2a PE"
The specificity of staining by monoclonal antibodies to target antigens should be verified by establishing the amount of non-specific antibody binding. Especially at higher concentration (more than 15μ/ml) the antibody staining usually has consignable background. To this end a non-reactive immunoglobulin of the same isotype is included as a negative control for each specific monoclonal antibody used in a particular immunoassay. The monoclonal antibody PPV-04, generated against a plant pathogen, does not cross-react with other species, and hence all the background that could be observed when working with this antibody would be a result of general nonspecific interactions between an mouse IgG2a molecule and the respective sample under the particular conditions. This shall help the customer to set up the experimental conditions so that the nonspecific binding of any antibody is abolished.
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