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CyFlow™ CD5 Biotin

CyFlow™ CD5 Biotin
Antigen: CD5
Alternative Name: Leu-1, T1
Clone: L17F12
Application: Flow cytometry
Format/Fluorochrome: Biotin
Target Species: Human
Field of Interest: Immunophenotyping
Species of Origin: Mouse
Regulatory Status: RUO
Clonality: monoclonal
Isotype: IgG2a
Order number: BH589141

For Research Use Only
Not for use in diagnostic or therapeutic procedures.

HLDA Workshop HLDA II Concentration Unit mg/mL Concentration 1 Quantity 0.1 mg... more
CyFlow™ CD5 Biotin
HLDA WorkshopHLDA II
Concentration Unitmg/mL
Concentration1
Quantity0.1 mg
Volume0.1
ImmunogenHuman Acute Lymphocytic Leukemia (ALL) T cells
Background InformationCD5 (T1) is a human cell surface T-lymphocyte single-chain transmembrane glycoprotein. CD5 is expressed on all mature T-lymphocytes, most of thymocytes, subset of B-lymphocytes and on many T-cell leukemias and lymphomas. It is a type I membrane glycoprotein whose extracellular region contains three scavenger receptor cysteine-rich (SRCR) domains. The CD5 is a signal transducing molecule whose cytoplasmic tail is devoid of any intrinsic catalytic activity. CD5 modulates signaling through the antigen-specific receptor complex (TCR and BCR). CD5 crosslinking induces extracellular Ca++ mobilization, tyrosine phosphorylation of intracellular proteins and DAG production. Preliminary evidence shows protein associations with ZAP-70, p56lck, p59fyn, PC-PLC, etc. CD5 may serve as a dual receptor, giving either stimulatory or inhibitory signals depending both on the cell type and development stage. In thymocytes and B1a cells seems to provide inhibitory signals, in peripheral mature T lymhocytes it acts as a costimulatory signal receptor. CD5 is the phenotypic marker of a B cell subpopulation involved in the production of autoreactive antibodies. Disease relevance: CD5 is a phenotypic marker for some B cell lymphoproliferative disorders (B-CLL, Hairy cell leukemia, etc.). The CD5+ popuation is expanded in some autoimmune disorders (Rheumatoid Arthritis, etc.). Herpes virus infections induce loss of CD5 expression in the expanded CD8+ human T cells.
UsageThe reagent is designed for indirect immunofluorescence analysis by Flow Cytometry. Working concentrations should be determined by the investigator.
Storage BufferThe reagent is provided in phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.

Specific References

| Engleman EG, Warnke R, Fox RI, Dilley J, Benike CJ, Levy R: Studies of a human T lymphocyte antigen recognized by a monoclonal antibody. Proc·Natl·Acad·Sci·USA. 1981·Mar; 78(3):1791‑5. <·PMID:·7015346·> | Shuster JJ, Falletta JM, Pullen DJ, Crist WM, Humphrey GB, Dowell BL, Wharam MD, Borowitz M: Prognostic factors in childhood T‑cell acute lymphoblastic leukemia: a Pediatric Oncology Group study. Blood. 1990·Jan·1; 75(1):166‑73. <·PMID:·1688495·> | McAlister MS, Davis B, Pfuhl M, Driscoll PC: NMR analysis of the N‑terminal SRCR domain of human CD5: engineering of a glycoprotein for superior characteristics in NMR experiments. Protein·Eng. 1998·Oct; 11(10):847‑53. <·PMID:·9862202·> | Gong JZ, Lagoo AS, Peters D, Horvatinovich J, Benz P, Buckley PJ: Value of CD23 determination by flow cytometry in differentiating mantle cell lymphoma from chronic lymphocytic leukemia/small lymphocytic lymphoma. Am·J·Clin·Pathol. 2001·Dec; 116(6):893‑7. <·PMID:·11764079·> | Dunphy CH, Tang W: The value of CD64 expression in distinguishing acute myeloid leukemia with monocytic differentiation from other subtypes of acute myeloid leukemia: a flow cytometric analysis of 64 cases. Arch·Pathol·Lab·Med. 2007·May; 131(5):748‑54. <·PMID:·17488160·>