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CyFlow™ CD10 PE

CyFlow™ CD10 PE
Antigen: CD10
Alternative Name: CALLA, gp100, NEP
Clone: MEM-78
Application: Flow cytometry
Format/Fluorochrome: PE
Laser: Blue, Green, Yellow
Target Species: Human
Field of Interest: Immunophenotyping
Species of Origin: Mouse
Regulatory Status: RUO
Clonality: monoclonal
Emission Maximum: 576 nm
Excitation Maximum: 496 nm, 565 nm
Isotype: IgG1
Order number: BQ465424

For Research Use Only
Not for use in diagnostic or therapeutic procedures.

HLDA Workshop HLDA IV—WS Code B 506 HLDA V—WS Code B CD10.4 Quantity 100 tests Volume... more
CyFlow™ CD10 PE
HLDA WorkshopHLDA IV—WS Code B 506 HLDA V—WS Code B CD10.4
Quantity100 tests
Volume2
ImmunogenNALM-6 human pre-B cell line
Background InformationCD10 (neutral endopeptidase - NEP, common acute lymphocytic leukemia antigen - CALLA, membrane metallo-endopeptidase - MME, enkefalinase) is a 100 kDa cell surface zinc metalloprotease cleaving peptide bonds on the N-terminus of hydrophobic amino acids and inactivating multiple physiologically active peptides. CD10 is expressed on various normal cell types, including lymphoid precursor cells, germinal center B lymhocytes, and some epithelial cells, and its expression level serves as a marker for diagnostics of many carcinomas. CD10 is also a differentiation antigen for early B-lymphoid progenitors in the B-cell differentiation pathway and has a key role in regulation of growth, differentiation and signal transduction of many cellular systems.
UsageThe reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 20·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.
Storage BufferThe reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.

Specific References

| Horejsí V, Angelisová P, Bazil V, Kristofová H, Stoyanov S, Stefanová I, Hausner P, Vosecký M, Hilgert I: Monoclonal antibodies against human leucocyte antigens (II); Antibodies against CD45 (T200), CD3 (T3), CD43, CD10 (CALLA), transferrin receptor (T9), a novel broadly expressed 18‑kDa antigen (MEM‑43) and a novel antigen of restricted expression (MEM‑74). Folia·Biol·(Praha). 1988; 34(1):23‑34. <·PMID:·2968928·> | Knapp W, Dorken B, Gilks W, Rieber EP, Schmidt RE, Stein H, von dem Borne AEGK (Eds): Leucocyte Typing IV. Oxford·University·Press,·Oxford. 1989; 1‑1820. <·NLM·ID:·8914679·> | Doussis IA, Gatter KC, Mason DY: CD68 reactivity of non‑macrophage derived tumours in cytological specimens. J·Clin·Pathol. 1993·Apr; 46(4):334‑6. <·PMID:·7684403·> | Angelisová P, Drbal K, Cerny J, Hilgert I, Horejsi V: Characterization of the human leukocyte GPI‑anchored glycoprotein CDw108 and its relation to other similar molecules. Immunobiology. 1999·Jun; 200(2):234‑45. <·PMID:·10416131·> | Suzuki T, Ino K, Kikkawa F, Uehara C, Kajiyama H, Shibata K, Mizutani S: Neutral endopeptidase/CD10 expression during phorbol ester‑induced differentiation of choriocarcinoma cells through the protein kinase C‑ and extracellular signal‑regulated kinase‑dependent signalling pathway. Placenta. 2002·Jul; 23(6):475‑82. <·PMID:·12137745·> | Yada K, Kashima K, Daa T, Kitano S, Fujiwara S, Yokoyama S: Expression of CD10 in basal cell carcinoma. Am·J·Dermatopathol. 2004·Dec; 26(6):463‑71. <·PMID:·15618927·> | Braham H, Trimeche M, Ziadi S, Mestiri S, Mokni M, Amara K, Hachana M, Sriha B, Korbi S: CD10 expression by fusiform stromal cells in nasopharyngeal carcinoma correlates with tumor progression. Virchows·Arch. 2006·Aug; 449(2):220‑4. <·PMID:·16673118·> | Dall'Era MA, True LD, Siegel AF, Porter MP, Sherertz TM, Liu AY: Differential expression of CD10 in prostate cancer and its clinical implication. BMC·Urol. 2007·Mar·2; 7:3. <·PMID:·17335564·>