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CyFlow™ CD15 Pacific Blue™
| Application: | Flow cytometry |
| Isotype: | IgM |
| Alternative Name: | 3-fucosyl-N-acetyllactosamine, Le(x), Lewis X, SSEA-1 |
| Field of Interest: | Blood group antigens, Immunophenotyping |
| Clone: | MEM-158 |
| Antigen: | CD15 |
| Emission Maximum: | 455 nm |
| Clonality: | monoclonal |
| Excitation Maximum: | 400 to 410 nm |
| Laser: | Violet |
| Format/Fluorochrome: | Pacific Blue™ |
| Source Species: | Mouse |
| Regulatory Status: | RUO |
| Target Species: | Human |
| Order number: | BS939780 |
For Research Use Only
Not for use in diagnostic or therapeutic procedures.
HLDA Workshop HLDA VI - WS Code AS A053 Quantity 100 tests Volume 1.0 mL Immunogen... more
Product information "CyFlow™ CD15 Pacific Blue™"
| HLDA Workshop | HLDA VI - WS Code AS A053 |
| Quantity | 100 tests |
| Volume | 1.0 mL |
| Immunogen | Human granulocytes |
| Background Information | CD15 (Le(x); Lewis x or SSEA-1; stage specific embryonic antigen-1) is a trisacharide determinant (3-fucosyl-N-acetyllactosamine) expressed on several glycolipids, glycoproteins and proteoglycans of various cell types, e.g. granulocytes, mast cells, monocytes, macrophages, cells of gastric mucosa, nervous system or various tumor cells. There are several variants of Lewis x, such as sialyl-Lewis x or sulphated Lewis x. Cells with high surface expression of Le(x) antigen exhibit strong self-aggregation, based on calcium-dependent Le(x)-Le(x) interaction. This process is involved for example in embryo compaction or in autoaggregation of teratocarcinoma cells. Sialyl-Le(x) and its isomer sialyl-Le(a) are ligands of selectins. CD15 expression has been extensively used to confirm diagnosis of Hodgkin´s disease. |
| Usage | The reagent is designed for flow cytometry analysis of human blood cells. Recommended usage is 4 µl reagent / 100 µl of whole blood or 10^6 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests. |
| Storage Buffer | The reagent is provided in stabilizing MES buffered saline solution, pH ≈5.5, containing 0.09% (w/v) sodium azide and 0.2% (w/v) BSA. |
| Storage | Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze. |
| Stability | Do not use after expiration date stamped on vial label. |
Safety Data Sheet
Technical Data Sheet
- CyFlow CD15 Pacific Blue (MEM-158) BS939780 TDS RUO.pdf
- CyFlow CD15 Pacific Blue (MEM-158) BS022461 TDS ASR EN.pdf
- CyFlow CD15 Pacific Blue (MEM-158) BS022461 TDS ASR EN.pdf
Certificate Of Analysis
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