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CyFlow™ CD15 FITC
Application: | Flow cytometry |
Clonality: | monoclonal |
Format/Fluorochrome: | FITC |
Target Species: | Human |
Regulatory Status: | RUO |
Laser: | Blue |
Excitation Maximum: | 490 to 495 nm |
Emission Maximum: | 518 nm |
Source Species: | Mouse |
Field of Interest: | Blood group antigens, Immunophenotyping |
Isotype: | IgM |
Antigen: | CD15 |
Alternative Name: | Lewis X |
Clone: | MEM-158 |
Order number: | CM349973 |
For Research Use Only
Not for use in diagnostic or therapeutic procedures.
HLDA Workshop HLDA VI - WS Code AS A053 Quantity 100 tests Volume 2.0 mL Immunogen... more
Product information "CyFlow™ CD15 FITC"
HLDA Workshop | HLDA VI - WS Code AS A053 |
Quantity | 100 tests |
Volume | 2.0 mL |
Immunogen | Human granulocytes |
Background Information | CD15 (Le(x); Lewis x or SSEA-1; stage specific embryonic antigen-1) is a trisacharide determinant (3-fucosyl-N-acetyllactosamine) expressed on several glycolipids, glycoproteins and proteoglycans of various cell types, e.g. granulocytes, mast cells, monocytes, macrophages, cells of gastric mucosa, nervous system or various tumor cells. There are several variants of Lewis x, such as sialyl-Lewis x or sulphated Lewis x. Cells with high surface expression of Le(x) antigen exhibit strong self-aggregation, based on calcium-dependent Le(x)-Le(x) interaction. This process is involved for example in embryo compaction or in autoaggregation of teratocarcinoma cells. Sialyl-Le(x) and its isomer sialyl-Le(a) are ligands of selectins. CD15 expression has been extensively used to confirm diagnosis of Hodgkin´s disease. |
Usage | The reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 20·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests. |
Storage Buffer | The reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide and 0.2% (w/v) BSA. |
Storage | Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze. |
Stability | Do not use after expiration date stamped on vial label. |
Safety Data Sheet
Technical Data Sheet
Certificate Of Analysis
- CyFlow CD15 FITC BA723310 COA 902039.pdf
- CyFlow CD15 FITC BA723310 COA 904013.pdf
- CyFlow CD15 FITC AT515824 COA 904013.pdf
- CyFlow CD15 FITC AT515824 COA 905305.pdf
- CyFlow CD15 FITC BA723310 COA 905305.pdf
- CyFlow CD15 FITC AT515824 COA 906527.pdf
- CyFlow CD15 FITC BA723310 COA 906527.pdf
- CyFlow CD15 FITC AT515824 COA 907593.pdf
- CyFlow CD15 FITC BA723310 COA 907593.pdf
ECDoC
Instruction for Use
- CyFlow CD15 FITC (MEM-158) AT515824 IFU CZ CZ.pdf
- CyFlow CD15 FITC (MEM-158) AT515824 IFU DE DE.pdf
- CyFlow CD15 FITC (MEM-158) AT515824 IFU DK DK.pdf
- CyFlow CD15 FITC (MEM-158) AT515824 IFU ES ES.pdf
- CyFlow CD15 FITC (MEM-158) AT515824 IFU FR FR.pdf
- CyFlow CD15 FITC (MEM-158) AT515824 IFU GR EL.pdf
- CyFlow CD15 FITC (MEM-158) AT515824 IFU IT IT.pdf
- CyFlow CD15 FITC (MEM-158) AT515824 IFU NL NL.pdf
- CyFlow CD15 FITC (MEM-158) AT515824 IFU NO NO.pdf
- CyFlow CD15 FITC (MEM-158) AT515824 IFU PL PL.pdf
- CyFlow CD15 FITC (MEM-158) AT515824 IFU PT PT.pdf
- CyFlow CD15 FITC (MEM-158) AT515824 IFU RU RU.pdf
- CyFlow CD15 FITC (MEM-158) AT515824 IFU SE SV.pdf
- CyFlow CD15 FITC (MEM-158) AT515824 IFU SK SK.pdf
- CyFlow CD15 FITC (MEM-158) AT515824 IFU TR TR.pdf
- CyFlow CD15 FITC (MEM-158) AT515824 IFU GB EN.pdf
- CyFlow CD15 FITC (MEM-158) AT515824 IFU BG BG.pdf
- CyFlow CD15 FITC (MEM-158) AT515824 IFU HR HR.pdf
- CyFlow CD15 FITC (MEM-158) AT515824 IFU RO RO.pdf
- CyFlow CD15 FITC (MEM-158) AT515824 IFU SI SL.pdf
- CyFlow CD15 FITC (MEM-158) AT515824 IFU RS SR.pdf
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