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CyFlow™ CD15 Purified
Application: | Flow cytometry, Immunoprecipitation |
Clonality: | monoclonal |
Target Species: | Human |
Regulatory Status: | RUO |
Source Species: | Mouse |
Field of Interest: | Blood group antigens, Immunophenotyping |
Format/Fluorochrome: | Purified |
Isotype: | IgM |
Antigen: | CD15 |
Alternative Name: | Lewis X |
Clone: | MEM-158 |
Order number: | AA641237 |
For Research Use Only
Not for use in diagnostic or therapeutic procedures.
HLDA Workshop HLDA VI - WS Code AS A053 Concentration Unit mg/mL Concentration 1... more
Product information "CyFlow™ CD15 Purified"
HLDA Workshop | HLDA VI - WS Code AS A053 |
Concentration Unit | mg/mL |
Concentration | 1 |
Quantity | 0.1 mg |
Volume | 0.1 mL |
Immunogen | Human granulocytes |
Background Information | CD15 (Le(x); Lewis x or SSEA-1; stage specific embryonic antigen-1) is a trisacharide determinant (3-fucosyl-N-acetyllactosamine) expressed on several glycolipids, glycoproteins and proteoglycans of various cell types, e.g. granulocytes, mast cells, monocytes, macrophages, cells of gastric mucosa, nervous system or various tumor cells. There are several variants of Lewis x, such as sialyl-Lewis x or sulphated Lewis x. Cells with high surface expression of Le(x) antigen exhibit strong self-aggregation, based on calcium-dependent Le(x)-Le(x) interaction. This process is involved for example in embryo compaction or in autoaggregation of teratocarcinoma cells. Sialyl-Le(x) and its isomer sialyl-Le(a) are ligands of selectins. CD15 expression has been extensively used to confirm diagnosis of Hodgkin´s disease. |
Storage Buffer | The reagent is provided in Tris buffered saline (TBS) solution, pH ≈8.0, containing 0.09% (w/v) sodium azide. |
Storage | Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze. |
Stability | Do not use after expiration date stamped on vial label. |
Safety Data Sheet
Technical Data Sheet
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