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CyFlow™ CD16 Alexa Fluor™ 647
| Application: | Flow cytometry |
| Clonality: | monoclonal |
| Target Species: | Human, Non-Human Primates |
| Regulatory Status: | RUO |
| Source Species: | Mouse |
| Field of Interest: | Immunophenotyping |
| Laser: | Red |
| Excitation Maximum: | 650 nm |
| Isotype: | IgG1 |
| Format/Fluorochrome: | Alexa Fluor™ 647 |
| Emission Maximum: | 665 nm |
| Antigen: | CD16 |
| Alternative Name: | FcγRIII |
| Clone: | 3G8 |
| Order number: | BR245099 |
For Research Use Only
Not for use in diagnostic or therapeutic procedures.
HLDA Workshop HLDA V—WS Code NK80 Quantity 100 tests Volume 0.4 mL Immunogen... more
Product information "CyFlow™ CD16 Alexa Fluor™ 647"
| HLDA Workshop | HLDA V—WS Code NK80 |
| Quantity | 100 tests |
| Volume | 0.4 mL |
| Immunogen | Human neutrophils |
| Background Information | CD16 (FcγRIII) is a 50-65 kDa glycoprotein serving as a low affinity IgG receptor. Human FcγRIII is expressed in two forms - FcγRIIIA and FcγRIIIB. FcγRIIIA is a transmembrane protein of monocytes, macrophages, NK cells and a subset of T cells. It is associated with FcεRI-γ subunit and is responsible for antibody-dependent NK cell cytotoxicity. Mast cell FcγRIIIA is associated, moreover, with FcεRI-β subunit. Besides IgG, FcγRIIIA can be triggered also by oligomeric IgE. FcγRIIIB is a GPI-linked monomeric receptor expressed on neutrophils and is involved in their activation and induction of a proadhesive phenotype. |
| Usage | The reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 4·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests. |
| Storage Buffer | The reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide and 0.2% (w/v) BSA. |
| Storage | Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze. |
| Stability | Do not use after expiration date stamped on vial label. |
Safety Data Sheet
Technical Data Sheet
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