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CyFlow™ CD19 PE-Cy7

CyFlow™ CD19 PE-Cy7
Application: Flow cytometry
Clonality: monoclonal
Target Species: Human
Regulatory Status: RUO
Laser: Blue, Green, Yellow
Source Species: Mouse
Field of Interest: Immunophenotyping
Excitation Maximum: 496 nm, 565 nm
Isotype: IgG1
Format/Fluorochrome: PE-Cy7
Emission Maximum: 785 nm
Antigen: CD19
Alternative Name: B4
Clone: 4G7
Order number: BJ745739

For Research Use Only
Not for use in diagnostic or therapeutic procedures.

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Description
Quantity 100 tests Volume 0.4 mL Immunogen Human Chronic Lymphocytic Leukemia (CLL)... more
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Product information "CyFlow™ CD19 PE-Cy7"
Quantity100 tests
Volume0.4 mL
ImmunogenHuman Chronic Lymphocytic Leukemia (CLL) cells
Background InformationCD19 is a transmembrane glycoprotein of Ig superfamily expressed by B cells from the time of heavy chain rearrangement until plasma cell differentiation. It forms a tetrameric complex with CD21 (complement receptor type 2), CD81 (TAPA-1) and Leu13. Together with BCR (B cell antigen receptor), this complex signals to decrease B cell treshold for activation by the antigen. Besides being signal-amplifying coreceptor for BCR, CD19 can also signal independently of BCR coligation and it turns out to be a central regulatory component upon which multiple signaling pathways converge. Mutation of the CD19 gene results in hypogammaglobulinemia, whereas CD19 overexpression causes B cell hyperactivity.
UsageThe reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 4·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Storage BufferThe reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide and 0.2% (w/v) BSA.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.
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