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CyFlow™ CD20 Pacific Blue™
| Application: | Flow cytometry |
| Excitation Maximum: | 400 to 410 nm |
| Clone: | 2H7 |
| Alternative Name: | B1, Bp35 |
| Antigen: | CD20 |
| Target Species: | Human, Non-Human Primates |
| Emission Maximum: | 455 nm |
| Laser: | Violet |
| Clonality: | monoclonal |
| Format/Fluorochrome: | Pacific Blue™ |
| Field of Interest: | Immunophenotyping, Signaling |
| Source Species: | Mouse |
| Isotype: | IgG2b |
| Regulatory Status: | RUO |
| Order number: | AP890385 |
For Research Use Only
Not for use in diagnostic or therapeutic procedures.
HLDA Workshop HLDA IV—WS Code B201 Quantity 100 tests Volume 0.4 mL Immunogen... more
Product information "CyFlow™ CD20 Pacific Blue™"
| HLDA Workshop | HLDA IV—WS Code B201 |
| Quantity | 100 tests |
| Volume | 0.4 mL |
| Immunogen | Human tonsillar B cells |
| Background Information | CD20 (B1, Bp35) is a cell surface 33-37 (depending on the degree of phosphorylation) kDa non-glycosylated surface phosphoprotein expressed on mature and most malignant B cells, but not stem cells or plasma cells (low number of the CD20 has been also detected on a subpopulation of T lymphocytes and it can be expressed on follicular dendritic cells). Its expression on B cells is synchronous with the expression of surface IgM. CD20 regulates transmembrane calcium conductance (probably functioning as a component of store-operated calcium channel), cell cycle progression and B-cell proliferation. It is associated with lipid rafts, but the intensity of this association depends on extracellular triggering, employing CD20 conformational change and/or BCR (B cell antigen receptor) aggregation. After the receptor ligation, BCR and CD20 colocalize and then rapidly dissociate before BCR endocytosis, whereas CD20 remains at the cell surface. CD20 serves as a useful target for antibody-mediated therapeutic depletion of B cells, as it is expressed at high levels on most B-cell malignancies, but does not become internalized or shed from the plasma membrane following mAb treatment. |
| Usage | The reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 4·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests. |
| Storage Buffer | The reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide and 0.2% (w/v) BSA. |
| Storage | Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze. |
| Stability | Do not use after expiration date stamped on vial label. |
Safety Data Sheet
Technical Data Sheet
- CyFlow™ CD20 Pacific Blue™ (2H7) AQ315269 TDS US,CA EN.pdf
- CyFlow CD20 Pacific Blue (2H7) AP890385 TDS RUO.pdf
- CyFlow CD20 Pacific Blue (LT20) CE562401 TDS RUO.pdf
- CyFlow CD20 Pacific Blue (2H7) AQ315269 TDS ASR EN.pdf
Instruction for Use
- CyFlow CD20 Pacific Blue (2H7) AM264349 IFU CZ CZ.pdf
- CyFlow CD20 Pacific Blue (2H7) AM264349 IFU DK DK.pdf
- CyFlow CD20 Pacific Blue (2H7) AM264349 IFU GR EL.pdf
- CyFlow CD20 Pacific Blue (2H7) AM264349 IFU IT IT.pdf
- CyFlow CD20 Pacific Blue (2H7) AM264349 IFU NL NL.pdf
- CyFlow CD20 Pacific Blue (2H7) AM264349 IFU NO NO.pdf
- CyFlow CD20 Pacific Blue (2H7) AM264349 IFU PL PL.pdf
- CyFlow CD20 Pacific Blue (2H7) AM264349 IFU RU RU.pdf
- CyFlow CD20 Pacific Blue (2H7) AM264349 IFU SE SV.pdf
- CyFlow CD20 Pacific Blue (2H7) AM264349 IFU SK SK.pdf
- CyFlow CD20 Pacific Blue (2H7) AM264349 IFU TR TR.pdf
- CyFlow CD20 Pacific Blue (2H7) CG435901 IFU CZ CZ.pdf
- CyFlow CD20 Pacific Blue (2H7) AM264349 IFU DE DE.pdf
- CyFlow CD20 Pacific Blue (2H7) AM264349 IFU ES ES.pdf
- CyFlow CD20 Pacific Blue (2H7) AM264349 IFU FR FR.pdf
- CyFlow CD20 Pacific Blue (2H7) AM264349 IFU GB EN.pdf
- CyFlow CD20 Pacific Blue (2H7) AM264349 IFU PT PT.pdf
- CyFlow CD20 Pacific Blue (2H7) CG435901 IFU DE DE.pdf
- CyFlow CD20 Pacific Blue (2H7) CG435901 IFU DK DK.pdf
- CyFlow CD20 Pacific Blue (2H7) CG435901 IFU ES ES.pdf
- CyFlow CD20 Pacific Blue (2H7) CG435901 IFU FR FR.pdf
- CyFlow CD20 Pacific Blue (2H7) CG435901 IFU GB EN.pdf
- CyFlow CD20 Pacific Blue (2H7) CG435901 IFU GR EL.pdf
- CyFlow CD20 Pacific Blue (2H7) CG435901 IFU IT IT.pdf
- CyFlow CD20 Pacific Blue (2H7) CG435901 IFU NL NL.pdf
- CyFlow CD20 Pacific Blue (2H7) CG435901 IFU NO NO.pdf
- CyFlow CD20 Pacific Blue (2H7) CG435901 IFU PL PL.pdf
- CyFlow CD20 Pacific Blue (2H7) CG435901 IFU PT PT.pdf
- CyFlow CD20 Pacific Blue (2H7) CG435901 IFU RU RU.pdf
- CyFlow CD20 Pacific Blue (2H7) CG435901 IFU SE SV.pdf
- CyFlow CD20 Pacific Blue (2H7) CG435901 IFU SK SK.pdf
- CyFlow CD20 Pacific Blue (2H7) CG435901 IFU TR TR.pdf
- CyFlow CD20 Pacific Blue (2H7) CG435901 IFU BG BG.pdf
- CyFlow CD20 Pacific Blue (2H7) CG435901 IFU HR HR.pdf
- CyFlow CD20 Pacific Blue (2H7) CG435901 IFU RO RO.pdf
- CyFlow CD20 Pacific Blue (2H7) CG435901 IFU SI SL.pdf
- CyFlow CD20 Pacific Blue (2H7) CG435901 IFU RS SR.pdf
ECDoC
Certificate Of Analysis
- CyFlow CD20 Pacific Blue AM264349 COA 901943.pdf
- CyFlow CD20 Pacific Blue AQ315269 COA 901943.pdf
- CyFlow CD20 Pacific Blue AQ315269 COA 903605.pdf
- CyFlow CD20 Pacific Blue AM264349 COA 903605.pdf
- CyFlow CD20 Pacific Blue CG435901 COA 903605.pdf
- CyFlow CD20 Pacific Blue AQ315269 COA 905107.pdf
- CyFlow CD20 Pacific Blue CG435901 COA 905107.pdf
- CyFlow CD20 Pacific Blue AQ315269 COA 906265.pdf
- CyFlow CD20 Pacific Blue CG435901 COA 906265.pdf
- CyFlow CD20 Pacific Blue CG435901 COA 906486.pdf
- CyFlow CD20 Pacific Blue AQ315269 COA 906486.pdf
- CyFlow CD20 Pacific Blue CG435901 COA 907596.pdf
- CyFlow CD20 Pacific Blue AQ315269 COA 907596.pdf
- CyFlow CD20 Pacific Blue AQ315269 COA 908409.pdf
- CyFlow CD20 Pacific Blue CG435901 COA 908409.pdf
- CyFlow CD20 Pacific Blue AQ315269 COA 909185.pdf
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