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CyFlow™ CD20 PerCP

CyFlow™ CD20 PerCP
Application: Flow cytometry
Format/Fluorochrome: PerCP
Clone: 2H7
Alternative Name: B1, Bp35
Antigen: CD20
Emission Maximum: 678 nm
Excitation Maximum: 482 to 490 nm
Target Species: Human, Non-Human Primates
Clonality: monoclonal
Laser: Blue, Green
Field of Interest: Immunophenotyping, Signaling
Source Species: Mouse
Isotype: IgG2b
Regulatory Status: RUO
Order number: AF735192

For Research Use Only
Not for use in diagnostic or therapeutic procedures.

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Description TDS & SDS
Description
HLDA Workshop HLDA IV—WS Code B201 Quantity 100 tests Volume 1.0 mL Immunogen... more
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Product information "CyFlow™ CD20 PerCP"
HLDA WorkshopHLDA IV—WS Code B201
Quantity100 tests
Volume1.0 mL
ImmunogenHuman tonsillar B cells
Background InformationCD20 (B1, Bp35) is a cell surface 33-37 (depending on the degree of phosphorylation) kDa non-glycosylated surface phosphoprotein expressed on mature and most malignant B cells, but not stem cells or plasma cells (low number of the CD20 has been also detected on a subpopulation of T lymphocytes and it can be expressed on follicular dendritic cells). Its expression on B cells is synchronous with the expression of surface IgM. CD20 regulates transmembrane calcium conductance (probably functioning as a component of store-operated calcium channel), cell cycle progression and B-cell proliferation. It is associated with lipid rafts, but the intensity of this association depends on extracellular triggering, employing CD20 conformational change and/or BCR (B cell antigen receptor) aggregation. After the receptor ligation, BCR and CD20 colocalize and then rapidly dissociate before BCR endocytosis, whereas CD20 remains at the cell surface. CD20 serves as a useful target for antibody-mediated therapeutic depletion of B cells, as it is expressed at high levels on most B-cell malignancies, but does not become internalized or shed from the plasma membrane following mAb treatment.
UsageThe reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 10·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
Storage BufferThe reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide and 0.2% (w/v) BSA.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.
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ECDoC

Certificate Of Analysis

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