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CyFlow™ CD25 PE-Cy7
| Application: | Flow cytometry |
| Isotype: | IgG1 |
| Alternative Name: | IL2Rα, p55, TAC |
| Antigen: | CD25 |
| Emission Maximum: | 785 nm |
| Format/Fluorochrome: | PE-Cy7 |
| Excitation Maximum: | 496 nm, 565 nm |
| Clonality: | monoclonal |
| Laser: | Blue, Green, Yellow |
| Field of Interest: | Immunophenotyping |
| Source Species: | Mouse |
| Regulatory Status: | RUO |
| Target Species: | Human |
| Clone: | MEM-181 |
| Order number: | BM796015 |
For Research Use Only
Not for use in diagnostic or therapeutic procedures.
HLDA Workshop HLDA VI—WS Code NL N-L024 Quantity 100 tests Volume 0.4 mL Immunogen... more
Product information "CyFlow™ CD25 PE-Cy7"
| HLDA Workshop | HLDA VI—WS Code NL N-L024 |
| Quantity | 100 tests |
| Volume | 0.4 mL |
| Immunogen | PHA-activated peripheral blood leucocytes |
| Background Information | CD25 (IL2Rα, Tac) is a ligand-binding α subunit of interleukin 2 receptor (IL2R). Together with β and γ subunit CD25 constitues the high affinity IL2R, whereas CD25 alone serves as the low affinity IL2R. CD25 expression rapidly increases upon T cell activation. The 55 kDa CD25 molecule is enzymatically cleaved and shed from the cell surface as a soluble 45 kDa s-Tac, whose concentration in serum can be used as a marker of T cell activation. Expression of CD25 indicates the neoplastic phenotype of mast cells. Humanized anti CD25 antibodies represent a useful tool to reduce the incidence of allograft rejection as well as the severity of graft versus host reaction, and radioimmunoconjugates of anti-CD25 antibodies can be used against CD25 expressing lymphomas. |
| Usage | The reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 4·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests. |
| Storage Buffer | The reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide and 0.2% (w/v) BSA. |
| Storage | Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze. |
| Stability | Do not use after expiration date stamped on vial label. |
Safety Data Sheet
Technical Data Sheet
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