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CyFlow™ CD45 APC

CyFlow™ CD45 APC
Application: Flow cytometry
Field of Interest: Immunophenotyping, MHC, Phosphorylation
Alternative Name: B220, LCA, T200
Antigen: CD45
Source Species: Rat
Isotype: IgG
Clonality: monoclonal
Target Species: Mouse
Emission Maximum: 660 nm
Excitation Maximum: 650 nm
Laser: Red
Format/Fluorochrome: APC
Regulatory Status: RUO
Clone: EM-05
Order number: AY699401

For Research Use Only
Not for use in diagnostic or therapeutic procedures.

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Description
Concentration Unit mg/mL Concentration 0,5 Quantity 0.1 mg Volume 0.2 mL... more
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Product information "CyFlow™ CD45 APC"
Concentration Unitmg/mL
Concentration0,5
Quantity0.1 mg
Volume0.2 mL
ImmunogenMouse peripheral blood leukocytes
Background InformationCD45 (LCA; leukocyte common antigen) is a receptor-type protein tyrosine phosphatase ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms as a result of alternative splicing; these isoforms differ in their extracellular domains, whereas they share identical transmembrane and cytoplasmic domains. These isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. Besides the role in immunoreceptor signaling, CD45 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis.
UsageThe reagent is designed for Flow Cytometry analysis. Suggested working usage is 4·µg/ml. Indicated dilution is recommended starting point for use of this product, but working concentrations should be validated by the investigator.
Storage BufferThe reagent is provided in phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.
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