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CyFlow™ CD45R0 PE-Cy7

CyFlow™ CD45R0 PE-Cy7
Application: Flow cytometry
Excitation Maximum: 496 nm, 565 nm
Alternative Name: UCHL-1
Antigen: CD45R0
Field of Interest: Immunophenotyping, Phosphorylation
Isotype: IgG2a
Emission Maximum: 785 nm
Format/Fluorochrome: PE-Cy7
Laser: Blue, Green, Yellow
Clonality: monoclonal
Source Species: Mouse
Regulatory Status: RUO
Target Species: Human
Clone: UCHL1
Order number: CS876696
HLDA Workshop HLDA III—WS Code NL 826 HLDA III—WS Code T 128 HLDA IV—WS Code N 31 HLDA V—WS... more
Product information "CyFlow™ CD45R0 PE-Cy7"
HLDA WorkshopHLDA III—WS Code NL 826 HLDA III—WS Code T 128 HLDA IV—WS Code N 31 HLDA V—WS Code BP BP460 HLDA V—WS Code T T-081 HLDA V—WS Code T T-CD45.43
Quantity100 tests
Volume0.4 mL
ImmunogenHuman IL-2 dependent T cells
Background InformationCD45R0 (UCHL-1) is the shortest isoform of a receptor-type protein tyrosine phosphatase, CD45 glycoprotein. CD45 is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases, promotes cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. CD45 isoforms differ in their extracellular domains, whereas they share identical transmembrane and cytoplasmic domains. These isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. CD45R0 is expressed e.g. on macrophages, CD8+ T cells, activated T cells and myeloma cells.
UsageThe reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 4·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Storage BufferThe reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide and 0.2% (w/v) BSA.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.
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