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CyFlow™ CD54 PE

CyFlow™ CD54 PE
Antigen: CD54
Alternative Name: ICAM-1
Clone: MEM-111
Application: Flow cytometry
Format/Fluorochrome: PE
Laser: Blue, Green, Yellow
Target Species: Cow, Human, Rat
Field of Interest: Immunophenotyping
Species of Origin: Mouse
Regulatory Status: RUO
Clonality: monoclonal
Emission Maximum: 576 nm
Excitation Maximum: 496 nm, 565 nm
Isotype: IgG2a
Order number: AD327626

For Research Use Only
Not for use in diagnostic or therapeutic procedures.

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Order 2, get the lower priced for free

*1
EUROBIOTECH2017

Order two antibodies and get the lower-priced one for free

*1 Offer valid until 23/09/2017
HLDA Workshop HLDA VI—WS Code AS A049 Quantity 100 tests Volume 2 Immunogen Raji... more
CyFlow™ CD54 PE
HLDA WorkshopHLDA VI—WS Code AS A049
Quantity100 tests
Volume2
ImmunogenRaji cells: human Burkitt's lymphoma cell line
Background InformationCD54 (ICAM-1) is a 90 kD member of the C2 subset of immunoglobulin superfamily. It is a transmembrane molecule with 7 potential N-glycosylated sites, expressed on resting monocytes and endothelial cells and can be upregulated on many other cells, e.g. with lymphokines, on B- and T-lymphocytes, thymocytes, dendritic cells and also on keratinocytes, chondrocytes, as well as epithelial cells. CD54 mediates cell adhesion by binding to integrins CD11a/CD18 (LFA-1) and to CD11b/CD18 (Mac-1). The interaction of CD54 with LFA-1 enhances antigen-specific T-cell activation.
UsageThe reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 20·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.
Storage BufferThe reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.

Specific References

| Leeuwenberg JF, Smeets EF, Neefjes JJ, Shaffer MA, Cinek T, Jeunhomme TM, Ahern TJ, Buurman WA: E‑selectin and intercellular adhesion molecule‑1 are released by activated human endothelial cells in vitro. Immunology. 1992·Dec; 77(4):543‑9. <·PMID:·1283598·> | Kishimoto T, Goyert S, Kikutani H, Mason D, Miyasaka M, Moretta L, Ohno T, Okumura K, Shaw S, Springer TA, Sugamura K, Sugawara H, von dem Borne AEGK, Zola H (Eds): Leucocyte Typing VI. Garland·Publishing·Inc,·New·York. 1997; 1‑1342. <·NLM·ID:·9712219·> | Nagaraju K, Raben N, Merritt G, Loeffler L, Kirk K, Plotz P: A variety of cytokines and immunologically relevant surface molecules are expressed by normal human skeletal muscle cells under proinflammatory stimuli. Clin·Exp·Immunol. 1998; 113(3):407‑14. <·PMID:·9737670·> | Bacáková L, Mares V, Bottone MG, Pellicciari C, Lisá V, Svorcík V: Fluorine ion‑implanted polystyrene improves growth and viability of vascular smooth muscle cells in culture. J·Biomed·Mater·Res. 2000·Mar·5; 49(3):369‑79. <·PMID:·10602070·> | Bacáková L, Mares V, Lysá V, Svorcík V: Molecular mechanisms of improved adhesion and growth of an endothelial cell line cultured on polystyrene implanted with fluorine ions. Biomaterials. 2000·Jun; 21(11):1173‑9. <·PMID:·10817270·> | Tachimoto H, Kikuchi M, Hudson SA, Bickel CA, Hamilton RG, Bochner BS: Eotaxin‑2 Alters Eosinophil Integrin Function via Mitogen‑Activated Protein Kinases. Am·J·Respir·Cell·Mol·Biol·. 2002; 26:645. <·PMID:·12034562·> | Hertel L, Lacaille VG, Strobl H, Mellins ED, Mocarski ES: Susceptibility of Immature and Mature Langerhans Cell‑Type Dendritic Cells to Infection and Immunomodulation by Human Cytomegalovirus. J·Virol. 2003; 77(13):7563‑74. <·PMID:·12805456·> | Burdick MM, McCaffery JM, Kim YS, Bochner BS, Konstantopoulos K: Colon carcinoma cell glycolipids, integrins, and other glycoproteins mediate adhesion to HUVECs under flow. Am·J·Physiol·Cell·Physiol·284,·C977·(2003).. 2003·Apr; 284(4):C977‑87. <·PMID:·12477667·> | Lee DJ, Sieling PA, Ochoa MT, Krutzik SR, Guo B, Hernandez M, Rea TH, Cheng G, Colonna M, Modlin RL: LILRA2 activation inhibits dendritic cell differentiation and antigen presentation to T cells. J·Immunol. 2007·Dec·15; 179(12):8128‑36. <·PMID:·18056355·>

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