Welcome to
Sysmex Flow Cytometry Other European Country!

For ordering please make sure you are in your local shop.
If you are from Other European Country then please continue.
Otherwise please go to your country.

Go to my country Continue

Article successfully added.

CyFlow™ CD54 PE

CyFlow™ CD54 PE
Antigen: CD54
Alternative Name: ICAM-1
Clone: MEM-111
Application: Flow cytometry
Format/Fluorochrome: PE
Laser: Blue, Green, Yellow
Target Species: Cow, Human, Rat
Field of Interest: Immunophenotyping
Species of Origin: Mouse
Regulatory Status: RUO
Clonality: monoclonal
Emission Maximum: 576 nm
Excitation Maximum: 496 nm, 565 nm
Isotype: IgG2a
Order number: AD327626

For Research Use Only
Not for use in diagnostic or therapeutic procedures.

HLDA Workshop HLDA VI—WS Code AS A049 Quantity 100 tests Volume 2 Immunogen Raji... more
CyFlow™ CD54 PE
HLDA WorkshopHLDA VI—WS Code AS A049
Quantity100 tests
ImmunogenRaji cells: human Burkitt's lymphoma cell line
Background InformationCD54 (ICAM-1) is a 90 kD member of the C2 subset of immunoglobulin superfamily. It is a transmembrane molecule with 7 potential N-glycosylated sites, expressed on resting monocytes and endothelial cells and can be upregulated on many other cells, e.g. with lymphokines, on B- and T-lymphocytes, thymocytes, dendritic cells and also on keratinocytes, chondrocytes, as well as epithelial cells. CD54 mediates cell adhesion by binding to integrins CD11a/CD18 (LFA-1) and to CD11b/CD18 (Mac-1). The interaction of CD54 with LFA-1 enhances antigen-specific T-cell activation.
UsageThe reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 20·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.
Storage BufferThe reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.

Specific References

| Leeuwenberg JF, Smeets EF, Neefjes JJ, Shaffer MA, Cinek T, Jeunhomme TM, Ahern TJ, Buurman WA: E‑selectin and intercellular adhesion molecule‑1 are released by activated human endothelial cells in vitro. Immunology. 1992·Dec; 77(4):543‑9. <·PMID:·1283598·> | Kishimoto T, Goyert S, Kikutani H, Mason D, Miyasaka M, Moretta L, Ohno T, Okumura K, Shaw S, Springer TA, Sugamura K, Sugawara H, von dem Borne AEGK, Zola H (Eds): Leucocyte Typing VI. Garland·Publishing·Inc,·New·York. 1997; 1‑1342. <·NLM·ID:·9712219·> | Nagaraju K, Raben N, Merritt G, Loeffler L, Kirk K, Plotz P: A variety of cytokines and immunologically relevant surface molecules are expressed by normal human skeletal muscle cells under proinflammatory stimuli. Clin·Exp·Immunol. 1998; 113(3):407‑14. <·PMID:·9737670·> | Bacáková L, Mares V, Bottone MG, Pellicciari C, Lisá V, Svorcík V: Fluorine ion‑implanted polystyrene improves growth and viability of vascular smooth muscle cells in culture. J·Biomed·Mater·Res. 2000·Mar·5; 49(3):369‑79. <·PMID:·10602070·> | Bacáková L, Mares V, Lysá V, Svorcík V: Molecular mechanisms of improved adhesion and growth of an endothelial cell line cultured on polystyrene implanted with fluorine ions. Biomaterials. 2000·Jun; 21(11):1173‑9. <·PMID:·10817270·> | Tachimoto H, Kikuchi M, Hudson SA, Bickel CA, Hamilton RG, Bochner BS: Eotaxin‑2 Alters Eosinophil Integrin Function via Mitogen‑Activated Protein Kinases. Am·J·Respir·Cell·Mol·Biol·. 2002; 26:645. <·PMID:·12034562·> | Hertel L, Lacaille VG, Strobl H, Mellins ED, Mocarski ES: Susceptibility of Immature and Mature Langerhans Cell‑Type Dendritic Cells to Infection and Immunomodulation by Human Cytomegalovirus. J·Virol. 2003; 77(13):7563‑74. <·PMID:·12805456·> | Burdick MM, McCaffery JM, Kim YS, Bochner BS, Konstantopoulos K: Colon carcinoma cell glycolipids, integrins, and other glycoproteins mediate adhesion to HUVECs under flow. Am·J·Physiol·Cell·Physiol·284,·C977·(2003).. 2003·Apr; 284(4):C977‑87. <·PMID:·12477667·> | Lee DJ, Sieling PA, Ochoa MT, Krutzik SR, Guo B, Hernandez M, Rea TH, Cheng G, Colonna M, Modlin RL: LILRA2 activation inhibits dendritic cell differentiation and antigen presentation to T cells. J·Immunol. 2007·Dec·15; 179(12):8128‑36. <·PMID:·18056355·>