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CyFlow™ CD58 Biotin

CyFlow™ CD58 Biotin
Application: Flow cytometry
Clonality: monoclonal
Target Species: Human, Pig
Regulatory Status: RUO
Source Species: Mouse
Field of Interest: Immunophenotyping
Isotype: IgG1
Format/Fluorochrome: Biotin
Antigen: CD58
Alternative Name: LFA-3
Clone: MEM-63
Order number: CE076390
HLDA Workshop HLDA VI—WS Code AS A047 Concentration Unit mg/mL Concentration 1... more
Product information "CyFlow™ CD58 Biotin"
HLDA WorkshopHLDA VI—WS Code AS A047
Concentration Unitmg/mL
Concentration1
Quantity0.1 mg
Volume0.1 mL
ImmunogenNALM-6 human pre-B cell line
Background InformationCD58 (LFA-3) is an immunoglobulin family adhession molecule expressed by both hematopoietic and non-hematopoietic cells (often on antigen presenting cells) and serving as ligand of CD2. This interaction is important for T cell-mediated immunity. CD58 is expressed in transmembrane form and in GPI-anchored form; the later is constitutively associated with protein kinases whereas the transmembrane form activates kinase activity upon triggering. CD58 is a powerful tool for detection of minimal residual disease in acute lymphocytic leukemia, and for evaluation of liver damage related with hepatitis B.
UsageThe reagent is designed for indirect immunofluorescence analysis by Flow Cytometry. Suggested working usage is 5·µg/ml. Indicated dilution is recommended starting point for use of this product, but working concentrations should be validated by the investigator.
Storage BufferThe reagent is provided in Tris buffered saline (TBS) solution, pH ≈8.0, containing 0.09% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.
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