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CyFlow™ CD62P PE

CyFlow™ CD62P PE
Application: Flow cytometry
Laser: Blue, Green, Yellow
Alternative Name: P-selectin
Antigen: CD62P
Target Species: Human, Non-Human Primates
Isotype: IgG1
Emission Maximum: 576 nm
Excitation Maximum: 496 nm, 565 nm
Clonality: monoclonal
Format/Fluorochrome: PE
Field of Interest: Immunophenotyping
Source Species: Mouse
Regulatory Status: RUO
Clone: AK4
Order number: AM736744
HLDA Workshop HLDA VI—WS Code P-44 Quantity 100 tests Volume 2.0 mL Immunogen... more
Product information "CyFlow™ CD62P PE"
HLDA WorkshopHLDA VI—WS Code P-44
Quantity100 tests
Volume2.0 mL
ImmunogenHuman platelets
Background InformationCD62P (P-selectin) is an adhesion glycoprotein that is expressed on platelets and endothelial cells upon their activation. Interaction between CD62P and its mucin-like ligand PSGL-1 (P-selectin glycoprotein ligand-1) expressed on the microvilli of most leukocytes supports leukocyte rolling along postkapillary venules at the earliest time of inflammation. Both CD62P and PSGL-1 are extended glycoproteins that form homodimers. CD62P dimerization is probably mediated through interactions of the transmembrane domains and stabilizes leukocyte tethering and rolling, probably by increasing rebinding within a bond cluster.
UsageThe reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 20·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.
Storage BufferThe reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide and 0.2% (w/v) BSA.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.
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