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CyFlow™ CD63 Biotin

CyFlow™ CD63 Biotin
Application: Flow cytometry
Clonality: monoclonal
Target Species: Human
Regulatory Status: RUO
Source Species: Mouse
Field of Interest: Endocytosis, Immunophenotyping
Isotype: IgG1
Format/Fluorochrome: Biotin
Antigen: CD63
Alternative Name: LAMP-3, LIMP, MLA1
Clone: MEM-259
Order number: CL410266
Concentration Unit mg/mL Concentration 1 Quantity 0.1 mg Volume 0.1 mL... more
Product information "CyFlow™ CD63 Biotin"
Concentration Unitmg/mL
Concentration1
Quantity0.1 mg
Volume0.1 mL
ImmunogenHuman Acute Lymphocytic Leukemia (ALL) T cells
Background InformationCD63 (LAMP-3; lysosome-associated membrane protein-3), a glycoprotein of tetraspanin family, is present in late endosomes, lysosomes and secretory vesicles of various cell types. It is also present in the plasma membrane, usually following cell activation. Hence, it has become an widely used basophil activation marker. In mast cells, however, CD63 exposition does not need their activation. CD63 interacts with integrins and affects phagocytosis and cell migration, it is also involved in H/K-ATPase trafficking regulation of ROMK1 channels. CD63 also serves as a T-cell costimulation molecule. Expression of CD63 can be used for predicting the prognosis in earlier stages of carcinomas.
UsageThe reagent is designed for indirect immunofluorescence analysis by Flow Cytometry. Working concentrations should be determined by the investigator.
Storage BufferThe reagent is provided in phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.
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