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CyFlow™ CD71 Alexa Fluor™ 647
| Application: | Flow cytometry |
| Format/Fluorochrome: | Alexa Fluor™ 647 |
| Alternative Name: | T9, TFR, TRFR |
| Antigen: | CD71 |
| Field of Interest: | Endocytosis, Immunophenotyping |
| Emission Maximum: | 665 nm |
| Isotype: | IgG1 |
| Clonality: | monoclonal |
| Excitation Maximum: | 650 nm |
| Laser: | Red |
| Source Species: | Mouse |
| Regulatory Status: | RUO |
| Target Species: | Human |
| Clone: | MEM-75 |
| Order number: | CX805345 |
For Research Use Only
Not for use in diagnostic or therapeutic procedures.
HLDA Workshop HLDA IV—WS Code A 045 HLDA V—WS Code T T-165 Quantity 100 tests Volume... more
Product information "CyFlow™ CD71 Alexa Fluor™ 647"
| HLDA Workshop | HLDA IV—WS Code A 045 HLDA V—WS Code T T-165 |
| Quantity | 100 tests |
| Volume | 0.4 mL |
| Immunogen | NALM-6 human pre-B cell line |
| Background Information | CD71 (transferrin receptor) is a type II transmembrane glycoprotein expressed as homodimer in erythroid blood cell line and in activated leukocytes. Upon binding of holotransferrin (complex of transferrin and iron ions), CD71 is internalized by clathrin-mediated endocytosis. Acidification of endosomes by vesicular membrane proton pumps leads to dissociation of iron ions, whereas transferrin (apotransferrin) remains associated with CD71 and recycles to the cell surface, where it is released upon exposure to normal pH. CD71 is also involved in uptake of non-transferrin bound iron. |
| Usage | The reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 4·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests. |
| Storage Buffer | The reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide and 0.2% (w/v) BSA. |
| Storage | Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze. |
| Stability | Do not use after expiration date stamped on vial label. |
Safety Data Sheet
Technical Data Sheet
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