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CyFlow™ CD72 PE

CyFlow™ CD72 PE
Application: Flow cytometry
Laser: Blue, Green, Yellow
Alternative Name: Lyb-2, LYB2
Antigen: CD72
Emission Maximum: 576 nm
Excitation Maximum: 496 nm, 565 nm
Format/Fluorochrome: PE
Clonality: monoclonal
Field of Interest: Immunophenotyping
Source Species: Mouse
Isotype: IgG2b
Regulatory Status: RUO
Target Species: Human
Clone: 3F3
Order number: AA664263
Quantity 100 tests Volume 2.0 mL Immunogen Normal human lymphocytes from lymph node... more
Product information "CyFlow™ CD72 PE"
Quantity100 tests
Volume2.0 mL
ImmunogenNormal human lymphocytes from lymph node
Background InformationCD72 is a transmembrane glycoprotein expressed as a homodimer especially in B cells, but also in other antigen presenting cells such as dendritic cells and macrophages. Through one of its immunoreceptor tyrosine-based inhibitory motives (ITIMs), CD72 interacts with tyrosine phosphatase SHP-1, thereby suppressing B cell responsiveness. Binding of CD72 with its ligand CD100 (Sema4D) prevents BCR association and phosphorylation of CD72 and results in dissociation of SHP-1 from CD72, thus enables B cell activation.
UsageThe reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 20·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.
Storage BufferThe reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide and 0.2% (w/v) BSA.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.
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