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CyFlow™ CD79b APC

CyFlow™ CD79b APC
Application: Flow cytometry
Clonality: monoclonal
Target Species: Human
Regulatory Status: RUO
Source Species: Mouse
Field of Interest: Immunophenotyping
Format/Fluorochrome: APC
Laser: Red
Excitation Maximum: 650 nm
Emission Maximum: 660 nm
Isotype: IgG1
Antigen: CD79b
Alternative Name: B29, IgB
Clone: CB3-1
Order number: AD554370
HLDA Workshop HLDA VI—WS Code CD79.1 Quantity 100 tests Volume 1.0 mL Immunogen... more
Product information "CyFlow™ CD79b APC"
HLDA WorkshopHLDA VI—WS Code CD79.1
Quantity100 tests
Volume1.0 mL
ImmunogenFraction of Ig-associated molecules isolated from Ramos B cells
Background InformationCD79b (IgB, B29) forms disulfide-linked heterodimer with CD79a (IgA, MB-1, MB1). They both are transmembrane proteins with extended cytoplasmic domains containing immunoreceptor tyrosine activation motives (ITAMs), and together with cell surface immunoglobulin they constitute B-cell antigen-specific receptor (BCR). CD79a and b are the first components of BCR that are expressed developmentally. They appear on pro-B cells in association with the endoplasmic reticulum chaperone calnexin. Subsequently, in pre-B cells, CD79 heterodimer is associated with λ5-VpreB surrogate immunoglobulin and later with antigen-specific surface immunoglobulins.
UsageThe reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 10·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
Storage BufferThe reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide and 0.2% (w/v) BSA.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.
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