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CyFlow™ CD86 FITC
| Application: | Flow cytometry |
| Clonality: | monoclonal |
| Format/Fluorochrome: | FITC |
| Regulatory Status: | RUO |
| Laser: | Blue |
| Excitation Maximum: | 490 to 495 nm |
| Emission Maximum: | 518 nm |
| Field of Interest: | Immunophenotyping, MHC |
| Isotype: | IgG2a |
| Target Species: | Mouse |
| Source Species: | Rat |
| Antigen: | CD86 |
| Alternative Name: | B7-2, B70 |
| Clone: | GL-1 |
| Order number: | CQ603647 |
For Research Use Only
Not for use in diagnostic or therapeutic procedures.
Concentration Unit mg/mL Concentration 0,5 Quantity 0.1 mg Volume 0.2 mL... more
Product information "CyFlow™ CD86 FITC"
| Concentration Unit | mg/mL |
| Concentration | 0,5 |
| Quantity | 0.1 mg |
| Volume | 0.2 mL |
| Immunogen | LPS-activated CBA/Cs mouse splenic B cells |
| Background Information | CD86 (B7-2) and CD80 (B7-1) are ligands of T cell critical costimulatory molecule CD28 and of an inhibitory receptor CD152 (CTLA-4). Both B7 molecules are expressed on professional antigen-presenting cells and are essential for T cell activation, both molecules can also substitute for each other in this process. The question what are the differences in CD80 and CD86 competency has not been fully elucidated yet; there are still conflicts in results about their respective roles in initiation or sustaining of the T cell immune response. |
| Usage | The reagent is designed for Flow Cytometry analysis. Suggested working usage is 2·µg/ml. Indicated dilution is recommended starting point for use of this product, but working concentrations should be validated by the investigator. |
| Storage Buffer | The reagent is provided in phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide. |
| Storage | Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze. |
| Stability | Do not use after expiration date stamped on vial label. |
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