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CyFlow™ CD86 Low Endotoxin

CyFlow™ CD86 Low Endotoxin
Antigen: CD86
Alternative Name: B7-2, B70
Clone: GL-1
Application: Flow cytometry, Functional assays, Immunocytochemistry, Immunohistochemistry (frozen sections), Immunoprecipitation
Format/Fluorochrome: Low Endotoxin
Target Species: Mouse
Field of Interest: Immunophenotyping, MHC
Species of Origin: Rat
Regulatory Status: RUO
Clonality: monoclonal
Isotype: IgG2a
Order number: BF446321

For Research Use Only
Not for use in diagnostic or therapeutic procedures.

Concentration Unit mg/mL Concentration 1 Quantity 0.1 mg Volume 0.1 Immunogen... more
CyFlow™ CD86 Low Endotoxin
Concentration Unitmg/mL
Concentration1
Quantity0.1 mg
Volume0.1
ImmunogenLPS-activated CBA/Cs mouse splenic B cells
Background InformationCD86 (B7-2) and CD80 (B7-1) are ligands of T cell critical costimulatory molecule CD28 and of an inhibitory receptor CD152 (CTLA-4). Both B7 molecules are expressed on professional antigen-presenting cells and are essential for T cell activation, both molecules can also substitute for each other in this process. The question what are the differences in CD80 and CD86 competency has not been fully elucidated yet; there are still conflicts in results about their respective roles in initiation or sustaining of the T cell immune response.
Storage BufferThe reagent is provided in azide-free phosphate buffered saline (PBS) solution, pH ≈7.4; 0.2 µm filter sterilized. Endotoxin level is less than 0.01 EU/µg of the protein, as determined by the LAL test.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.

Specific References

| Hathcock KS, Laszlo G, Dickler HB, Bradshaw J, Linsley P, Hodes RJ: Identification of an alternative CTLA‑4 ligand costimulatory for T cell activation. Science. 1993·Nov·5; 262(5135):905‑7. <·PMID:·7694361·> | Benschop RJ, Melamed D, Nemazee D, Cambier JC: Distinct signal thresholds for the unique antigen receptor‑linked gene expression programs in mature and immature B cells. J·Exp·Med. 1999·Sep·20; 190(6):749‑56. <·PMID:·10499913·> | Brasel K, De Smedt T, Smith JL, Maliszewski CR: Generation of murine dendritic cells from flt3‑ligand‑supplemented bone marrow cultures. Blood. 2000·Nov·1; 96(9):3029‑39. <·PMID:·11049981·> | Chung JB, Wells AD, Adler S, Jacob A, Turka LA, Monroe JG: Incomplete activation of CD4 T cells by antigen‑presenting transitional immature B cells: implications for peripheral B and T cell responsiveness: implications for peripheral B and T cell responsiveness. J·Immunol. 2003·Aug·15; 171(4):1758‑67. <·PMID:·12902475·> | Steptoe RJ, Ritchie JM, Jones LK, Harrison LC: Autoimmune diabetes is suppressed by transfer of proinsulin‑encoding Gr‑1+ myeloid progenitor cells that differentiate in vivo into resting dendritic cells. Diabetes. 2005·Feb; 54(2):434‑42. <·PMID:·15677501·> | Nolan A, Weiden M, Kelly A, Hoshino Y, Hoshino S, Mehta N, Gold JA: CD40 and CD80/86 act synergistically to regulate inflammation and mortality in polymicrobial sepsis. Am·J·Respir·Crit·Care·Med. 2008·Feb·1; 177(3):301‑8. <·PMID:·17989345·> | Edgtton KL, Kausman JY, Li M, O'Sullivan K, Lo C, Hutchinson P, Yagita H, Holdsworth SR, Kitching AR: Intrarenal antigens activate CD4+ cells via co‑stimulatory signals from dendritic cells. J·Am·Soc·Nephrol. 2008·Mar; 19(3):515‑26. <·PMID:·18184859·> | Radhakrishnan S, Arneson LN, Upshaw JL, Howe CL, Felts SJ, Colonna M, Leibson PJ, Rodriguez M, Pease LR: TREM‑2 mediated signaling induces antigen uptake and retention in mature myeloid dendritic cells. J·Immunol. 2008·Dec·1; 181(11):7863‑72. <·PMID:·19017976·> | Nolan A, Kobayashi H, Naveed B, Kelly A, Hoshino Y, Hoshino S, Karulf MR, Rom WN, Weiden MD, Gold JA: Differential role for CD80 and CD86 in the regulation of the innate immune response in murine polymicrobial sepsis. PLoS·One. 2009·Aug·12; 4(8):e6600. <·PMID:·19672303·>